polymerase chain reaction(redirected from Annealing temperature)
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polymerase chain reaction
n. Abbr. PCR
A technique for amplifying DNA sequences in vitro by separating the DNA into two strands and incubating it with oligonucleotide primers and DNA polymerase. It can amplify a specific sequence of DNA as many as one billion times and is important in biotechnology, forensics, medicine, and genetic research.
pol′ymerase chain′ reac`tion
the laboratory production of numerous copies of a gene by separating the two strands of the DNA containing the gene segment, marking its location with a primer, and using a DNA polymerase to assemble a copy alongside each segment and continuously copy the copies. Abbr.: PCR
amplification en chaîne par polymérase