polymerase chain reaction

(redirected from Annealing temperature)
Also found in: Medical, Encyclopedia.

polymerase chain reaction

n. Abbr. PCR
A technique for amplifying DNA sequences in vitro by separating the DNA into two strands and incubating it with oligonucleotide primers and DNA polymerase. It can amplify a specific sequence of DNA as many as one billion times and is important in biotechnology, forensics, medicine, and genetic research.

pol′ymerase chain′ reac`tion



n.
the laboratory production of numerous copies of a gene by separating the two strands of the DNA containing the gene segment, marking its location with a primer, and using a DNA polymerase to assemble a copy alongside each segment and continuously copy the copies. Abbr.: PCR
Translations
amplification en chaîne par polymérase
References in periodicals archive ?
We also investigate how the annealing temperature affects the optical properties of n-Si/PEDOT:PSS structures by measuring the reflectance spectra.
As the annealing temperature is raised to 823 K, a wide plateau can be observed from the load-depth curve suggesting stress-induced martensitic transformation on the nanoscale.
Particle size remained nearly the same regardless of the annealing temperature. Films deposited on ZnS substrates have rod-shaped particles, whereas CdS/SnS films contain flake-shaped particles.
When the annealing temperature increases, the size of nanoparticles grows quickly.
The degree of crystallization of LiNi[O.sub.2] was enhanced at 500[degrees]C because the (104) reflection became stronger as the annealing temperature was increased.
Grain size of polysilicon layer which directly affects the resistivity of the layer is controlled by deposition temperature, dopant concentration and annealing temperature.
A capping system, the corresponding heater and a control electronics, which allows to set the annealing temperature and the annealing useful are monitored for complete package.
The reaction is now rapidly cooled to a temperature at or slightly below the annealing temperature or TM of our primers (here, for the sake of example, being 52[degrees]C) (annealing, C).
The eventual extent of deformation is a function of the annealing temperature and time.
The relatively low annealing temperature could make the nanorods ideal for use in heat-sensitive nanoelectronics, particularly for "gluing" together the stacked components of 3-D computer chips.
Systematic evaluation revealed that the terminator concentration and the annealing temperature in the base extension reaction were critical for the reduction of false-positive detection in SABER.
The annealing temperature is about equal to or greater than the glass transition of the polymer.