It includes not only the insertion of a gene into the cell for expression, but also gene silencing with RNA interference (RNAi) and antisense RNA
, aptamers (DNA or RNA polymers which bind to a protein target), and oncolytic viruses.
My lab has made pioneering contributions to the understanding of C9orf72 FTD/ALS, and reported the first structure of the repeat RNA, and the first description of both sense and antisense RNA
aggregates in patient brain.
Some specific topics examined include clinical application of RNAs for cancer therapy, selection of target sites in RNA for antisense agents, and natural antisense RNA
technology used synthetic strands of RNA to deactivate messenger RNA, effectively blocking a gene's activity and preventing production of a particular protein.
The corresponding digoxigenin-labeled antisense RNA
probes were prepared from pGEM-1 containing a BamH1-EcoR1 956-bp insert consisting of exon 3 and flanking intron sequences from the rat IGF-1 gene, and the pTRI-IGFR-human transcription template containing a 236-bp cDNA fragment of the human IGF-1R gene spanning exons 8-7 (Ambion, Austin, TX, USA).
One method, antisense RNA
technology, proved to be unreliable.
Frechet foresees wide application for the technique in delivering antisense RNA
, proteins or genes into cell interiors, and integrating with current methodologies.
When mRNA forms a duplex with a complementary antisense RNA
sequence, one of the consequences is the blocking of translation.
Prior to this advance, researchers used the less robust antisense RNA
technology to achieve the same goals.
They've also identified antisense RNA
that helps control plasmid replication in lactococci.
Anti-HIV-1 antisense RNA
was detected in all patients after 6 months and has been detected in 2 of the 5 subjects for periods up to 12 months.
covering broad applications in the field of nucleic acid technology and the second in Canada for antisense RNA