For example, orthologs of escapin found in the ink gland of other sea hares include dactylomelin-P in Aplysia dactylomela (Melo et al., 2000) and APIT (Aplysia punctata ink toxin), cyplasin-L, and cyplasin-S in A.
Cloning and biochemical characterization of AP1T, a new L-amino acid oxidase from Aplysia punctata. Toxicon 46: 479-489.
We examined metabolic rate, shell morphometry, and calcification in the sea hare Aplysia punctata under short-term exposure (19 days) to an extreme ocean acidification scenario (pH 7.3, ~2800 [micro]atm pC[O.sub.2]), along with a group held in control conditions (pH 8.1, ~344 [micro]atm pC[O.sub.2]).
Its congener Aplysia punctata (Cuvier, 1803) is common in the north Atlantic in shallow waters and the intertidal.
Comparison of the biochemical and enzymatic properties of escapin, Aplysia punctata ink toxin (APIT), and other LAAOs is instructive in understanding their relationships and functions.
Table 1 List of clones recovered and analyzed with suppression subtractive hybridization Clone Number of group BLASTn results E value clones C4 Human DNA clone RP11-100A16 0.049 12 F7 Aplysia punctata mRNA for Cyplasin le-60 1 S E7 Klebsiella pneumoniae SL032 plasmid 0.003 1 B8 Human chromosome 16 clone RP11- 0.095 1 407623 Clone Size of group fragment (bp) C4 278 F7 161 E7 635 B8 367 Acknowledgments