Stained sections were visualized with an Apotome
fluorescence microscope (Zeiss).
Samples were mounted on glass slides and cells were observed under a ZEISS ApoTome
.2 Fluorescent Microscope (ZEISS, Germany).
The following day, cell nuclei were counterstained with DAPI (Invitrogen, Grand Island, NY, USA) and mounted with mounting medium and images were captured digitally using either the Axio Imager Apotome
microscope (Zeiss, Gottingen, Germany) or the Confocal Laser Scanning microscope (Leica TCS SP8, Buffalo Grove, IL, USA).
Stained sections were analyzed using a Zeiss Z1 Apotome
microscope and Axiovision software.
To estimate the number of instars, the following measurements were taken: total body length (TL), lateral length of the head capsule (LLH, a line drawn from the base of the labral fan stalk to the posterior limit of the head, passing through the ocelli), and the width of the frontoclypeal apotome
(WFA) (as in Alencar et al.
The venue (which also serves as NATO's headquarters in Milan) will house a selection of older works, two site-specific commissions, and a trio of new videos, including Apotome
, the artists' haunting meditation on the psychological and sociocultural properties of the human voice.
Light Microscopy Group, Gottingen, Germany, and Carl Zeiss, Thornwood, N.Y., have developed the APOTOME
system to aid researchers conducting critical biomedical research.
is a 3D imaging system for contrast enhancement in fluorescence microscopy based on grid projection techniques using HBO light source.
The distinguishing characteristics for species are found in the shape of the asymmetrical notch on the anterior margin of the frontoclypeal apotome
. A summary of published distributional records is provided for species of associate larvae.