Several algal samples collected during different phases of its growth were tested on Artemia salina
and other model organisms from different taxa (other crustaceans, larvae and juveniles of sea urchins and polyps and ephyrae of jellyfish) to understand how toxic effects vary with direct/indirect exposures and life cycle (Faimali et al., 2012; Privitera et al., 2012; Giussani et al., 2016).
Culture of Artemia salina
.--The Artemia saline cysts were transferred to an aquarium containing synthetic saline solution (60 g of sea salt / liter of distilled water) and oxygen saturation, obtained with the aid of an air pump.
Lethality test: Brine shrimps (Artemia salina
) were used as a model to test feather extract toxicity.
Toxicity of carbamates to the brine shrimp Artemia salina
and the effect of atropine, BW284c51, iso-OMPA, and 2-PAM on carbaryl toxicity.
The micro well cytotoxicity assay against Artemia salina
showed the cytotoxicity of the extracts in the range of 10-35%.
In contrast, lower cytotoxicity (35%) was observed against brine shrimp (Artemia salina
) larvae after 24 hours of exposure.
Durante los primeros 10 dias posteclosion (DPE), las larvas se mantuvieron en incubadoras de acrilico de 200 litros y fueron alimentadas con Artemia salina
en racion de 600 nauplios/ml/larva/dia.
Afterwards, the antioxidant effect and the biopolymer toxic potential in Artemia salina
Aiming to minimize the use of animals in experiments, many companies producing natural products already use the plant toxicity test against Artemia salina
in more advanced research to find bioactive compounds.
From the above, this study aimed to analyze the quality of collected apitoxin from the collector filaments after beneficiation and to compare it with the apitoxin collected directly from collector's glass plate, considering moisture content, protein and toxicity through Artemia salina
Fumarprotocetraric acid showed low toxicity against Artemia salina
larvae ([LC.sub.50] > 0.54 mM) and murine fibroblasts ([IC.sub.50] 0.4 mM) .