As they strive to improve the efficiency of the chromosome-building strategy, Willard and his coworkers are trying to answer questions about the nature of the artificial chromosome
, such as whether its centromere is pure alpha-satellite DNA.
Assembly of contiguous bacterial artificial chromosome
(BAC) sequences and comparisons with corresponding genetic linkage maps have provided more precise estimates of physical to genetic distance ratios within gene-rich regions.
The contract includes gene discovery and BAC (bacterial artificial chromosome
) screening across the Interleukin-1 (IL-1) region.
An artificial chromosome
will again give you unprecedented power to alter cells.
No BAC or yeast artificial chromosome
clones span this region (Saji et al., 2001; Chen et al., 2002).
An essential tool for characterizing genomes is the availability of yeast artificial chromosome
(YAC) or BAC libraries containing large genomic DNA inserts.
These papers include the development of physical maps using yeast artificial chromosome
(YAC) clones and bacterial artificial chromosomes
(BACs) and the construction of a rice contig map through a combination of fingerprinting and hybridization.
Individual bacterial artificial chromosome
(BAC) clones were selected from a Children's Hospital Oakland Research Institute library for each target on the chromosome.
The performance principle of array-CGH is basically the same as m-CGH with the difference being the use of a platform-array containing immobilized DNA fragments cloned with various vectors (bacterial artificial chromosome
, yeast artificial chromosomes
or P1-derived artificial chromosome
), instead of metaphase chromosomes .
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Arrays, Protein Arrays, Very High Density Assays - Oligonucleotide Arrays, Second Generation Sequencing, Phage Display), Application (Allergies, Autoimmune Diseases, Cardiac Diseases, Infectious Disease Diagnostics, Oncology, Pregnancy & Fertility Testing), End Users (Academic Research Institutes, Clinical Research Organizations, Diagnostic Laboratories, Hospital, Pharmaceutical Companies) and Forecast 2017-2021 report at https://www.ihealthcareanalyst.com/report/multiplex-molecular-diagnostics-market/
Spontaneous chromosome doubling may occur via somatic cell fusion, endoreduplication, endomitosis and possibly many other mechanisms (Jensen, 1974) but due to low spontaneous doubling rates, artificial chromosome
doubling methods are required for application of haploids in breeding programs (Rober et al., 2005; Chang and Coe, 2009; Geiger, 2009).
Both groups created a new model of C9ORF72 disease by inserting the gene carrying a large number of the expanded repeats into mice using a bacterial artificial chromosome
, a genetic engineering tool specialized for carrying very large pieces of DNA.