From 37 KOH preparation positive cases 24(64.86%) cases showed aseptate
hyphae in KOH preparation from these 24 cases 11(52.38%) cases grew Aspergillus species and 4(19.04%) grew Fusarium species.
There were no mycorrhizal structures in roots of pigeonpea and no aseptate
hyphae were observed in pots without mycorrhizal inoculum.
Conidia forming columns in culture and hyaline, aseptate
, smooth, long oval or cylindrical, grayish green en masse, 6.7-8.5 x 2.6-3.3 (= 7.4+-0.5 x 2.9+-0.3) um.
To determine mycorrhizal colonization, we visually transected the slides at 400x magnification and recorded either the presence or absence of stained AMF structures (counting only aseptate
hyphae, vesicles, or arbuscules) for each view.
Filamentous fungi with aseptate
mycelia were identified as "zygomycete," with no further characterization as to genus.
Microconidia are short-cylindrical or ellipsoidal and aseptate
Histological examination of gastric biopsy showed areas of ulceration and necrosis with inflammatory granulation, within areas of necrosis are seen many broad aseptate
fungal hyphae of mucor (Figure 1).
Biopsy report of the lung tissue revealed necrotic lung parenchyma with broad aseptate
ribbon-like hyphae consistent with Mucor species.
Caption: Figure 3: Grocott-Gomori methenamine-silver (GMS) stained tissue section of resected lung from an 18-year-old with recent kidney transplant highlighting aseptate
hyphae morphologically resembling fungal class Zygomycetes (400x).
Histopathology showed a chronic necrotizing and suppurative granulomatous dermatitis with numerous coenocytic (aseptate
) hyphae (Fig.
conidiophores and mostly radiate heads with mono and biserriate sterigmata mounted on flask shaped vesicles.