The glands in HGPIN may lack a clearly visible basal cell layer
on hematoxylin-eosin (H&E), and immunohistochemical stains used to highlight basal cells (high-molecular-weight cytokeratin and p63) typically show disruption of this layer with patchy discontinuous staining.
The lining of odontogenic keratocyst is parakeratinized with lining which is 5-19 cells thick with columnar cells present in the basal cell layer
having a palisaded nuclei with a superficial corrugated layer ,connective tissue capsule show blood vessels and collagen fibres.
The presence of a single cell lining with complete absence of the basal cell layer
The use of p63 in our study was to confirm the presence or absence of basal cell layer
, and accordingly, the precise confirmative diagnosis will be more established.
GSTP1 expression in benign tissue is heterogeneous: it strongly stains healthy gland basal cell layer
, while it frequently shows a weaker positivity or even absence of staining in luminal cells (Figures 1 and 2).
The cells were arranged into well-demarcated islands and strands, which appeared to be raised from the basal cell layer
of the overlying epidermis and invaded into the underlying connective tissue (figure 3).
Infection with HPV is opportunistic, and the virus gains access to the basal cell layer
following micro-abrasions along the squamous epithelium.
These cells raise from the basal cell layer
through each of the layers of the epidermis--stratum spinosum, gradulosum and lastly the stratum corneum the outer most layers.
As the terminology indicates, these lesions arise from the basal cell layer
of the epidermis, and they can be further defined histologically as superficial, nodular, micronodular, infiltrating, or other subtypes.
The epidermis itself has four layers: the stratum corneum, the granular layer, the squamous cell layer and the basal cell layer
The basal cell layer
had the typical palisaded pattern [Fig.
There was an absence of melanocytes in the basal cell layer
, confirmed by special staining.