This pathway is modulated by B-cell lymphoma protein 2 (BCL-2) family and proteins including proapoptotic proteins (BCL-2-associated X protein
(BAX), BCL-2 antagonist killer 1 (BAK), BCL-2 antagonist of cell death (BAD), BH3 interacting-domain death agonist (BID), etc.) and antiapoptotic proteins (BCL-2, BCL-2-related protein (BCL-XL), BCL-2-like 2 protein (BCL-W), myeloid cell leukemia-1 (MCL-1), etc.) .
Membranes were then blocked for 1 h at room temperature with Tris-buffered saline-Tween 20 (TBS-T; 20 mM Tris-HCl (pH7.4), 137.5 mM NaCl, and 0.1% Tween 20) containing 5% skim milk, washed with TBS-T three times, and probed at 4[degrees]C for 12 h with rabbit antibodies to sheep cytochrome c (Sigma-Aldrich), human Bcl-2 (1:500 dilution; Santa Cruz Biotechnology, Dallas, TX, USA), and human Bcl-2-associated X protein
(Bax; 1 : 500 dilution; Santa Cruz Biotechnology) or with goat antibodies to human [beta]-actin (1:500 dilution; Santa Cruz Biotechnology).