CHARACTERIZATION OF MULTIPLY GLYCOSYLATED (NT-) proBNP DERIVED PEPTIDES BY BEMAD AND NANO-LC ESI-[MS.
Ideally, BEMAD results in an exchange of the glycan of formerly glycosylated amino acids by a chemically more stable surrogate in collision-activated dissociation mass spectrometry.
Although the BEMAD treatment was incomplete at Ser44, 3 of the 4 sites revealed sequence-specific ions related to 2-mercaptoethanol adducts at positions Thr36, Ser37, and Thr48.
3] Nonstandard abbreviations: NT-proBNP, N-terminal pro-B-type natriuretic peptide (1-76); BNP, B-type natriuretic peptide (1-32); HF, heart failure; proBNP, pro-B-type natriuretic peptide (1-108); (NT-) proBNP, N-terminal pro-B-type natriuretic peptide (1-76)or proBNP (1-108); mAbs, monoclonal antibodies; IP, immunoprecipitation; pAb, polyclonal antibody; aa, amino acids; TIP, tandem immunoaffinity purification; [mu]-IA-HPLC, [mu]-immunoaffinity-HPLC; BEMAD, [beta]-elimination and Michael addition; nano-LC [ESIMS.
Produit par la compagnie [beaucoup moins que] BeMad
Production [beaucoup plus grand que], ce spectacle, programme pour la premiere fois en Algerie, prevoit 14 autres representations a Alger en collaboration avec l'Office national pour la culture et l'information, et ce, en ouverture de la [beaucoup moins que] saison [beaucoup plus grand que] de l'Opera d'Alger.
The author thanks Antonia Diaz for fruitful discussions and Jose Victor Rios-Rull, Tim Kehoe, Xavier Raurich, Javier Diaz-Jimenez, Juan Carlos Conesa, Patrick Toche and seminar participants at BEMAD
, the Carlos III Macroworkshop, Villa Mondragone Workshop, and the Universities of Modena, Malaga, and Basque Country for their comments
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