Total ammonia was monitored by colorimetric method with a Tetratest[R] Kit (Tetra Werke, Melle, Germany) and salinity with an optical refractometer F3000 (Bernauer Aquaculture, Blumenau, Brazil
During this procedure, asepsis was observed, including the use of lancets (Accu-Chek Safe-T-Pro Uno, Roche[R], Hawthorne, USA) and disposable gloves (Cremer[R], Blumenau, Brazil
Headquartered in Blumenau, Brazil
, Dental Cremer is the dental distribution business of Cremer S.A.
This rate is higher than that reported in the majority of Brazilian studies employing a similar methodology (2,5,8,22), but lower than the prevalence rate reported for the city of Blumenau, Brazil
Temperature was measured twice a day (7:00 am and 5:00 pm) and pH, dissolved oxygen, measured by a digital oximeter (YSI Bernauer, Company Bernauer, Blumenau, Brazil
) and ammonia every 7 day.
Sady Mazzioni Doctorate Student in Accountability and Administration by the Regional University of Blumenau, Brazil
Member of the Permanent Cientific Comission of the Monitoring Center of Research Groups and Scientific Initiation at Unichapeco
Wistar rats obtained from the Central Animal House of the Regional University of Blumenau, Blumenau, Brazil
, were used in the experiments.
Professor Florentin Smarandache from the University of New Mexico, United States, has deduced the existence of particles moving faster-than-light in a published paper called "There Is No Speed Barrier in the Universe" in 1998 , as an extension of a 1972 manuscript  that he also presented in 1993 at the conference Paradoxism in Literature and Science held in the Universidad de Blumenau, Brazil
. His paper is based on the Einstein-Podolsky-Rosen Paradox , a Bohm's paper , and Bell's Inequalities .