Samples extracted from the buffy coat
were diluted; 50 ng of DNA was added to each 20 [micro]L reaction and a nontemplate control (NTC) was included in each assay.
The Quantitative Buffy Coat
(QBC) technique involves the use of special fluorochrome dye to highlight malaria parasite at predictable location of a specially prepared capillary tube.
Blood parasites were not detected in wet blood film, Giemsa stained smears and buffy coat
Borrelia species were detected in Giemsa-stained thin blood smears by microscopy and quantitative buffy coat
analysis (Becton Dickinson, Le Pont de Claix, France) when available (4).
We obtained peripheral blood samples with informed consent and institutional approval from 10 pregnant women from each of the first (12-14 weeks), second (20-23 weeks), and third (38-40 weeks) trimesters and harvested the plasma and maternal buffy coat
from each case.
In the present study different modalities of malaria diagnosis which include the conventional peripheral smear examination with different staining techniques and the modern Quantitative buffy coat
test (QBC), rapid antigen detection testing are evaluated in clinically suspected cases.
Not that it isn't sufficiently, well, "clinical" to fit the bill; it is a rigorously scientific discussion of Quantitative Buffy Coat
Analysis hematology, and the science and technology related to it, by an outstanding physician who has had a parallel career as an inventor of such instrumentation and developer of ever-more innovative applications for it.
DNA from 17 chimpanzees (lung, spleen, or liver), 30 red colobus monkeys (buffy coat
, blood, bone marrow, intestine, spleen, liver, lung, or muscle), and 15 black-and-white colobus monkeys (buffy coat
, liver, intestine, or muscle) and 700 humans (blood), was prepared by using commercial DNA extraction kits (QIAGEN, Hilden, Germany).
The diagnosis is mainly based on blood smear and buffy coat
Here we utilize an emerging molecular quantification method called droplet digital PCR (ddPCR) to perform a ratio-based assay that rapidly and accurately detects ciHHV-6 from cellular samples, typically buffy coat
collected from whole blood.
(2) It was his invention and teachings, through multiple editions of his eponymous text Wintrobe's Clinical Hematology, promoting the visual examination of the buffy coat
of centrifuged blood, that led Wardlaw and me to invent, thirty-six years ago, the first dry hematology system that delivered multiple hematologic parameters.