A small incision was made between the eye and ear to expose the exorbital lacrimal gland followed by the application of 3 [micro]L of a 50 [micro]M carbamylcholine
solution (an analog of the parasympathetic agonist acetylcholine) topically to the gland, 3 times in 5-minute intervals.
In experiments, potassium chloride (KCl), histamine dihydrochloride, carbamylcholine
chloride (carbachol), hydroxyzine dihydrochloride, verapamil hydrochloride, and 2-pyridylethylamine were manually diluted in distilled water and added to the organ bath.
chloride, acetylcholine (ACh), and phenylephrine (Phe) were purchased from Sigma-Aldrich (USA).
Antinociceptive interactions with intravenous morphine and intrathecal clonidine, neostigmine, and carbamylcholine
We also noticed that muscarinic receptors could be solubilized in their active forms when porcine cerebral membranes were pre-treated with a muscarinic ligand such as carbamylcholine
or atropine, and then solubilized with sodium cholate in the presence of this ligand.
Chemicals and animals: Atropine sulphate, acetylcholine perchloride (ACh), verapamil hydrochloride, carbamylcholine
(CCh) and some constituents of chemicals utilized for producing physiological salt solutions and the reagents used in phytochemical analysis; magnesium chloride (MgCl2), potassium chloride (KCl), ethylenediamene-tetraacetic acid (EDTA), Dragedorff's reagent, ferric chloride (FeCl3), aluminium chloride (AlCl3), calcium chloride (CaCl2), and aluminium hydroxide (AlOH3) were acquired from Sigma-Aldrich Chemicals Company, St.