Roswell Park Memorial Institute medium (RPMI-1640), 96-well plates, Tween 20, Goat anti-mouse-peroxidase conjugate (IgG-HRP), substrate solution 3, 3',5,5'-tetramethylbenzidine (TMB), polyethylene glycol (PEG-1500), carbohydrates, bovine serum albumin (BSA), ovalbumin (OVA), phosphate buffer saline (PBS), hypoxanthine/thymidine/aminopterin (HAT) and fetal bovine serum (FBS) (Sigma-Aldrich and Thermo-Fischer Scientific, China) were used for cell cultures
Thus, numerous replicate 3D cell cultures
must be prepared to provide a robust dataset from which valid conclusions can be drawn.
They addressed topics on Introduction to the animal mammalian cell culture
lab, Demonstration on aseptic techniques, Preparation of media and other reagents, Principles of cell culture
techniques, Types of cell cultures
and applications, and Demonstration on chicken embryo and chicken embryo fibroblast culture.
The demand for cell culture
is rising due to continuous growth in the biologic drugs market such as vaccines, antibodies, and proteins using mammalian cell cultures
, production of artificial organs for use in regenerative medicine and increasing prevalence of cancer.
Stem cell researchers were specifically interested in documenting the generation of induced pluripotent stem cells via video recordings, while labs that grow cells under hypoxic or GMP conditions greatly appreciated the ability to remotely monitor their cell cultures
," said Herbert Mueller-Hartmann, head of R&D at Lonza Bioscience Solutions.
TAP Biosystems' RAFT 24-Well Kit can produce realistic, reproducible single or multiple 3D cell cultures
in physiologically relevant collagen scaffolds, and can improve the results of a range of cell-based assay models, including those suitable for in vitro toxicology models.
The line represents the first commercially-available bioreactors that support safe and cost-efficient large-scale (large-volume) production of traditional two-dimensional (2-D) cell cultures
with the advantage of cell culture
More complex than conventional cell cultures
, the devices can better replicate a living creature's bodily reactions.
Characterization of the Virus in EBN Primary Kidney Cell Cultures
The researchers compared osteopontin levels in a benchmark healthy ovarian epithelial cell culture
with those of 4 other healthy ovarian epithelial cell cultures
, 10 serous-type ovarian epithelial cancer cell lines, 2 mucinous-type ovarian cancer cell lines, and 2 clear cell-type ovarian cancer cell lines.
Additionally, the cell culture
should be immortal, but not tumorigenic, to avoid the complexities of human tissue acquisition that are inherent in the isolation and propagation of primary cell cultures
technology is used to carry out processes external to the natural environment by maintaining controlled environments which enable the growth of various cell cultures
for use in medicine and research.