Regulation (Alteration) of activity and conformation of sucrase by coaggregation with cellobiase
in culture medium of Termitomyces clypeatus.
After 10 days, crude enzyme was extracted by centrifugation and supernatant was analyzed for activities of carboxymethylcellulase, filterpaperasee and cellobiase
by Mandels et al (1976) method.
For the production of glucose a third type of activity, cellobiase
or [beta]-glucosidase (EC 126.96.36.199) is also required.
The enzymes, Cellulase (Celluclast 1.5L, Novozymes A/S, Denmark) and cellobiase
(Novozym 188, Novozymes A/S, Denmark) were added at 60 FPU per g glucan loading (FPU = filter paper unit) and 64 pNPGU per g glucan (pNPG = p-Nitrophenyl [beta]-D-glucoside), respectively and incubated for 72 hours.
Due to the feedback inhibition of reaction kinetics, the abundance of glucose affected the rate of cellobiose hydrolysis by cellobiase
(one of monoenzyme in the mixed cellulase).
This could be explained in terms of a poor cellobiase
activity inside the enzymatic cocktail.
Xia, "Production and immobilization of cellobiase
from Aspergillus niger ZU-07," Process Biochemistry, vol.
(2001) observed an increase in production of endoglucanase, filter paper activity and cellobiase
in two to fourfold of mutated T.
acts by cleaving the [beta]-1,4 glycosidic bond of cellobiose and small oligosaccharide molecules with release of monomeric sugars (WOOD; McCARE, 1979; BHAT, 2000).
Dekker, "Kinetic, inhibition, and stability properties of a commercial beta -d-glucosidase (cellobiase
) preparation from Aspergillus niger and its suitability in the hydrolysis of lignocellulose," Biotechnology and Bioengineering, vol.
4:15 EFFECTS OF TEMPERATURE, pH, AND SUBSTRATE CONCENTRATION ON CELLOBIASE
ACTIVITY FROM ENZYMES ISOLATED FROM COMMON AND GEORGIA-NATIVE MUSHROOMS **, Mary L.
Celluclast 1.5 L (cellulase) and Novozyme 188 (cellobiase
) were obtained from Novozymes Malaysia SdnBhd.