Direct ethanol production from cassava pulp using a surface-engineered yeast strain-co-displaying two amylases, two cellulases
, and a-glucosidase.
(EC 220.127.116.11) are responsible for hydrolysis of [beta] 1,4 linkage of cellulose to fermentable sugars which can be utilized as energy source (Dienes et al., 2004).
Isolation and Identification of New Cellulases
Producing Thermophilic Bacteria from an Egyptian Hot Spring and Some Properties of the Crude Enzyme.
All isolates of Bacillus amyloliquefaciens obtained were able to produce proteases, pectinases, cellulases
, and aamylases.
Many fungi and bacteria are capable of producing multiple enzymes, collectively known as cellulases
, that act in a synergistic manner to hydrolyze the [beta]-1,4-D-glycosidic bonds within the cellulose molecule (Akiba, Kimura, Yamamoto, & Kumagai, 1995).
The ability of growing in a wide range of carbon sources allows great variability in the production of cellulases
, since the gene expression and secretion of enzymes are directly dependent on the different chemical signals produced from the diverse substrates.
Experts all over the globe are seriously engaged in exploration of alternative food and energy sources out of which a promising source seems to be transformation of cellulosic wastes into sugars and alcohol by the enzymes cellulases
. The work reported in this article was carried to study characterization of cellulase
of Trichoderma viride.
However, there is limited information available for comparative analysis of silage prepared with these cellulases
produced from Acremonium and Trichoderma.
The production of xylanases and cellulases
is regulated by complex mechanisms of induction and repression, which depends upon the nature of the carbon source [10,11].
These enzymes include cellulases
, the main recruitable resource for the bioconversion of cellulosics to useful products, and usually the most costly part of the production process.
The strains belonging to the genus Trichosporum, Cryptococcus, Candida, Debaryomyces, Kluyveromyces have been described for producing cellulases
and xylanases constitutively [10, 11, 12], and also [beta]-glucosidases when grown in defined media inducers, such as cellobiose .
and chitinases have been discovered in a variety of crustaceans and molluscs, including the prawns Macrohrachium rosenbergii (de Man, 1879), Penaeus japonicas (Spence Bate, 1888), and Pandalus borealis (Kroyer, 1838) (Kono et al.