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Citraconylation used 0.1 mol/L citraconic anhydride in 1 mol/L N-methyl-morpholine, pH 9.0.
Another reagent, citraconic anhydride, which also modifies lysine residues, had little effect on the LX20 assay but decreased cross-reactivity in the Immulite assay by approximately 50%.
The olefinically unsaturated monomer corresponds to one or more monomers selected from at least one olefinically unsaturated monomer selected from the group consisting of: a) mono- and di-esters of crotonic acid, cinnamic acid, fumaric acid, maleic acid, citraconic
acid, and itaconic acid; b) carboxyfunctional monomers; c) N-vinyl caprolactam, vinyl phosphonates, N-vinyl formamides, N-vinyl acetamides, hydroxypropyl acrylates and methacrylates, monoacrylates and monomethacrylates of polyalkylene glycols, acrylates, methacrylates, acrylic acid and methacrylic acid amides containing amine groups; d) vinyl esters corresponding to general formula (I) CH2xCH--O--C(O)--R1, with R1=C11-21.