immunoprecipitation

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im•mu•no•pre•cip•i•ta•tion

(ˌɪm yə noʊ prɪˌsɪp ɪˈteɪ ʃən, ɪˌmyu-)

n.
the separation of an antigen from a solution by the formation of a large complex with its specific antibody.
[1965–70]
References in periodicals archive ?
Although the relative abundance of protein isotopologues will not be affected, reduced protein concentration due to lack of stability or removal of a protein target by coimmunoprecipitation could affect the ability to sensitively measure turnover.
Traditionally, piRNAs bound to PIWI proteins were identified by coimmunoprecipitation, followed by sequencing or RT-PCR of RNAs.
Coimmunoprecipitation experiments allowed us to demonstrate that NRP1 is able to bind KGF in ASCs.
Coimmunoprecipitation and colocalization experiments [105] revealed that TR[beta]1 and the PI3K p85[alpha] subunit were able to form a complex at the cytoplasmic level in both rRINm5F and hCM cell lines.
Moreover, coimmunoprecipitation revealed a physical interaction between pP90RSK and PER1, and inducible nuclear localization of pP90RSK was absent in [Per1.sup.ldc/ldc] mice [44].
In our study, the protein interaction between DJ-1 and NDUFS3 was first confirmed in rat testes by coimmunoprecipitation. Moreover, this interaction was reduced in AS.
Coimmunoprecipitation. Coimmunoprecipitation was performed for the determination of the interaction between Foxp3 and PPAR-[gamma] in [CD4.sup.+] T cells.
(90) Also, mass spectrometry analysis of the consensus TRPA1(386-405) peptide substrate treated with PHD2 and [O.sub.2], immunoblot analyses with the antibody raised against a synthetic TRPA1 subfragment carrying hydroxylated Pro394 as well as coimmunoprecipitation of TRPA1 with PHDs suggested that the TRPA1 protein is indeed hydroxylated by PHDs.
To confirm previous findings, we conducted coimmunoprecipitation assays to verify interactions between WT CIITA and WT pCAF.
Coimmunoprecipitation experiments confirmed that LRP1 interacts (directly or indirectly) with the IRp.
In line with this finding, several other researchers also reported direct binding of Nrf2 to newly identified AREs in the regions of the PPAR[gamma] promoter by using gel shift and coimmunoprecipitation assays (Figure 1) [48, 61, 64, 65].
The VP1-STK3 interaction identified by the yeast two-hybrid system was further confirmed by coimmunoprecipitation experiments and an indirect immunofluorescence assay (IFA).