Having said this, an unexplained phenomenon was evident in columns 5-10 of both the Cy5
and Cy3 blocks for all the slides used in which a gradual decrease in signal from column to column was evident in the columns under surveillance.
In the laboratory, each sample is labeled using red Cy5
and green Cy3 dyes.
On array 1 and array 3, the exercised muscles were incorporated with Cy3 dye, and on array 2 and array 4, exercised muscles were incorporated with Cy5
The lymphocyte population was indentified using anti-CD45 monoclonal antibody conjugated to Cy5
and integrated to establish the NK cell population using dual staining with anti-CD56 and CD16 monoclonal antibody conjugated to phycoerythrin (Beckman Coulter; Paris, France).
I would be very grateful for any information to Cede Nullis, 22 Les Cherfs, Castel, Guernsey, CY5
7HG or email margaret2186@hotmail.
Briefly, fluorescent RNA targets were prepared from 5 [micro]g of total RNA samples using the MessageAmp[TM] aRNA kit (Ambion, Austin, TX, USA) and Cy5
dye (Amersham Pharmacia, Piscataway, NJ, USA).
This technique involved pre-labeling two protein extracts, using two fluorescent cyanine dyes known as Cy3 and Cy5
Right) Images illustrate protein spots fluorescently labeled with Cy2, Cy3, or Cy5
EarlyTox Live Red Dye is detected by using Cy5
channel (Ex622/Em645 wavelength); EarlyTox Dead Green Dye is detected by using FITC channel (Ex503/Em526nm) wavelength.
The amplified HPV L1 gene and human beta actin gene were labeled with Cy5
and hybridized with an HPV DNA chip (GG HPV DNA Genotyping Chip Kit, GoodGene Inc, Seoul, Korea) that simultaneously detects 47 HPV genotypes.
Thus, the treatments were: control (non-amended sandy soil), CT (sandy soil+compost only), CT5 (sandy soil+a mixture of compost and 5% the fine-textured soil), CT20 (sandy soil + a mixture of compost and 20% the fine-textured soil), CY5
(sandy soil +5% fine-textured soil alone), CY20 (sandy soil + 20% fine-textured soil alone).
The control cDNA sample labeled with Cy5
and the treated sample cDNA labeled with Cy3 were combined and hybridized on the first microarray.