The samples were randomly labeled with Cy3 and Cy5
Al dia 6, el fenotipo de las CD inmaduras obtenidas se determino mediante citometria de flujo usando el siguiente panel de anticuerpos: CD14-V500 (BD Biosciences; Clon M5E2; catalogo 561391): HLADR-PerCP Cy5
A-Band fluorescence, excitation of the protonated chromophore of the fluorescent protein; B-Band fluorescence, excitation of the deprotonated chromophore form of the fluorescent protein: CFP, cyan fluorescent protein; Cy5
, cyanine dye; YFP, yellow fluorescent protein.
Cy2, Cy3, Cy5
, Deep Purple, pH 3-10 NL IPG strip (24cm),1,4-dithiothreitol (DTT), Acrylamide (Acr), carrier ampholyte, lysine, sodium dodecyl sulfate (SDS), N,N'-methylenebisacrylamide (Bis), 2D-Quant protein assay kit, thiourea and urea were obtained from GE Healthcare (US).
Whole blood (100 [micro]L) was stained with 10 [micro]L of CD45-phycoerythrin- (PE-) Cy5
antibody (clone J33) for 15 min at room temperature and protected from light.
The fluorescent signal generated by the internal control amplification is detected by a second probe labeled at the 5' end with a different reporter molecule, Cy5
through the Cy5
Bound autoantibody is detected with a fluorescent IRDye800-conjugated antihuman IgG antibody [simultaneous detection of IgG, IgA, and IgM antibodies was achieved with a combination of cyanine 3 (Cy3), IRDye800, and Cy5
Doll expects an 8% rise for the S&P this year, and noted in passing that "for 100 years, the stock market has always performed well" in years ending in Cy5
The amplified HPV L1 gene and human beta actin gene were labeled with Cy5
and hybridized with an HPV DNA chip (GG HPV DNA Genotyping Chip Kit, GoodGene Inc, Seoul, Korea) that simultaneously detects 47 HPV genotypes.
where SM was mean signal of four spots which replicated each probe when hybridized to cy5
labeled target and BM was mean background signal.
Thus, the treatments were: control (non-amended sandy soil), CT (sandy soil+compost only), CT5 (sandy soil+a mixture of compost and 5% the fine-textured soil), CT20 (sandy soil + a mixture of compost and 20% the fine-textured soil), CY5
(sandy soil +5% fine-textured soil alone), CY20 (sandy soil + 20% fine-textured soil alone).
I would be very grateful for any information to Cede Nullis, 22 Les Cherfs, Castel, Guernsey, CY5
7HG or email margaret2186@hotmail.