Moreover, the cytotoxicity (3T3 cell line) activity was carried out and cyclohexamide
was used as standard against normal cell line.
The explants were then cut into small pieces of 0.5 cm length with a flame sterilized scalpel and transferred to sterilized tryptic soy agar medium supplemented with antibiotic cyclohexamide
of 10 ppm concentration for the isolation of bacteria.
Similarly, under acidification, the relative effect of the inhibitor cyclohexamide
on metabolic rate of hepatocyte cells of Antarctic fish is depressed at pH 6.5 (vs.
They also found that cyclohexamide
blocked iodide uptake when added with TSH.
Cultures were obtained on Sabouraud dextrose agar supplemented with chloramphenicol and cyclohexamide
(to suppress bacteria and environmental fungi) and incubated at 26[degrees]C and 37[degrees]C.
experiments, 10 [micro]g/mL cyclohexamide
was added 1 hr before agonist treatment.
Gut sections for large insects or whole guts for small ones were macerated in 1mL of sterile PBS (phosphate buffered saline, pH 7.4) with a micropestle, and samples streaked aseptically onto two plates each of 1/10 Tryptic Soy Agar (Remel, Lenexa, KS) with 1% cyclohexamide
for bacteria or Rose Bengal Chloramphenicol Agar (BD, Franklin Lakes, N)) for fungi.
All specimens were subjected to direct microscopy for fungal elements in 10%/ 20% (for nail) KOH and culture in Sabouraud's Dextrose Agar (SDA) with chloramphenicol and cyclohexamide
Inhibitory agar such as those containing benomyl or cyclohexamide
can be useful in the differentiaton between P.
All samples were cultured in Sabouraud's dextrose agar with and without chloramphenicol and cyclohexamide
. Cultures were incubated for 30 days at room temperature.