DEAE cellulose


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Noun1.DEAE cellulose - used for chromatography
cellulose - a polysaccharide that is the chief constituent of all plant tissues and fibers
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The pooled fractions from gel filtration column exhibited the highest specific activity were exposed to an anion exchange chromatography using DEAE cellulose column (1.5x15cm) pre-equilibrated with the Tris-HCl buffer (50 mM; pH 7.5).
An attempt was made to separate cellulase components by binding with DEAE Cellulose' as anion exchanger.
Cellulases have the tendency of binding with the DEAE cellulose like other enzymes.
The supernatant was then applied on DEAE cellulose column previously equilibrated with 10 mM of Tris-HCl buffer pH 7.8.
Ion exchange chromatography: The dialyzed enzyme was loaded on to a column of DEAE cellulose (0.7 x 2.5 cm) equilibrated with 20mM Tris-HCl buffer (pH 8.5) and washed with same buffer.
The purity of the enzyme after DEAE cellulose chromatography was more than 27 fold, when compared to the crude extract of enzyme supernatant.
The traditional methods for estimating [HbA.sub.2] include elution after cellulose acetate electrophoresis and microcolumn chromatography using DEAE cellulose (DE-52).
Purification step Volume Protein Hemagglutinating (ml) (mg/ml) activity (HA) A- Crude lectins and salting out Whole serum (crude lectins) 25 2.28 512 70% saturated 150 0.62 256 [(N[H.sub.4]).sub.2] S[O.sub.4] B- DEAE cellulose chromatography Peak I 18 1.99 8 Peak II 24 1.73 16 Peak III 18 2.04 16 C- Affinity chromatography Pool 1 7.5 0.85 128 Pool 2 7.5 0.66 256 Poo13 6 0.55 256 Purification step Specific Yield (%) activity (HA/mg protein) A- Crude lectins and salting out Whole serum (crude lectins) 224.5 100 70% saturated 412.9 50 [(N[H.sub.4]).sub.2] S[O.sub.4] B- DEAE cellulose chromatography Peak I 4.02 1.562 Peak II 9.24 3.125 Peak III 7.84 3.125 C- Affinity chromatography Pool 1 150 25 Pool 2 387.8 50 Pool 3 465.4 50 Table 2.
Then sufficient amount of 0.5N NaOH was added to above treated DEAE cellulose in the same way five times and washed with distilled water so that maximum DEAE cellulose had settled down and its colour became white.
The DNA quality was further enhanced by purification using DEAE cellulose column chromatography.
aeruginosa using ammonium sulphate precipitation and DEAE cellulose column chromatography.