I digestion patterns shown in Figure 4 demonstrated that nuclear proteins from BC3H-1 myocytes treated for 3 hours with Hoechst 33342 (Figure 4, lane 3) and HL-60 cells treated for 6 hours with Hoechst 33342 (Figure 4, lane 5) specifically bound with the TTTGGCGC element, a known E2F-1-binding motif (Figure 4, lanes A and G).
I is most prevalent in mammals and is also found in bacteria, but not in low eukaryotes or plants.
1, aliquot #2 of each study participant) by rigorous DNase
treatment using a TURBO DNAfree[TM] kit (Applied Biosystems) followed by DNase
inactivation according to the manufacturer's instructions.
In order to eliminate contaminating protein and nucleic acids, treatment with proteinase K, DNase
and RNase was performed prior to extraction step.
There was no visible loss when treated with DNase
(data not shown).
One of them, an enzyme called DNase
I, appears less active in many lupus patients than in healthy people.
To date, genome-scale experiments by customers or Solexa include resequencing, gene expression, small RNA and specialty tag sequencing assays such as DNase
hypersensitivity detection and ChIP.
000k cleanroom environment via a fully automated process, which ensures they are free of external contaminants such as DNA, DNase
, RNase, ATP.
The Human DNA and DNase
Free swabs are sample-collection devices certified as free of human DNA contamination down to less than 25 picograms, and also certified to be DNase
expression allows the pathogen group A Streptococcus to escape killing in neutrophil extracellular traps.
Cell lysates were supplemented with DNase
I provided in the cells-to-cDNA II kit (0.
Crystal found that twice-daily inhalations of the drug, called DNase
I, improved the lung functions of all 16 of the adult subjects.