Thus, some authors believe that the endonuclease G released from mitochondria initiates DNA fragmentation and thereafter, this process of fragmentation is increased by DNase I
released from necrotic cells.
Characterization of human DNase I
family endonucleases and activation of DNase y during apoptosis.
Most commonly used for the testing of laboratory equipment, DNase I
non-specifically degrades double-stranded DNA.
Protein binding was noted in the extreme boundaries of this region, but not in an internal region previously described as both a topo II cleavage site and a DNase I
001 [micro]g of RNase A (Qiagen, Hilden, Germany) and 1 [micro]L (2 U) of Turbo DNA-free DNase I
(Ambion, Austin, TX, USA) with 1x Turbo DNA-free buffer were incubated at 37[degrees]C for 30 min under conditions that prevented destruction of viral RNA in the viral particles.
For another 10 samples, [beta]-globin DNA was not detected in any samples after we used on-column DNase digestion with 80 [micro]L of enzymebuffer mixture from the RNase-Free DNase Set (Qiagen) for the columnbased protocol and postextraction DNase treatment with a DNase I
reagent set (Invitrogen) for the automated protocol.
To the surface of their film, the Kagoshima researchers added their version of a self-destruct button: a negatively charged layer of the DNA-snipping enzyme DNase I
We have reported the presence of high DNase I
activity and its gene expression in the anterior lobe of the pituitary gland of both sexes and the abrupt increase in its gene expression at the onset of puberty (4).
One of them, an enzyme called DNase I
, appears less active in many lupus patients than in healthy people.
Crystal found that twice-daily inhalations of the drug, called DNase I
, improved the lung functions of all 16 of the adult subjects.
robust enough that it works in this procedure, even without the included buffer.
not as prevalent as RNase, but it is a concern of molecular biologists especially those doing polymerase chain reaction (PCR) with very small quantities of rare DNA.