Paraxylene is an aromatic hydrocarbon and isomer of xylene compound, which is derived from dimethylbenzene
. Paraxylene is widely used as a feedstock in the production of terephthalic acid (TPA),
The sections were baked overnight at 65 AdegC, dewaxed using dimethylbenzene
, and gradiently dehydrated using ethanol dehydration.
Sliced tissues were processed as follows: dewaxing and hydration; staining with hematoxylin for 10-20 min and rinsing for 1-3 min; differentiating with 1% hydrochloric acid for 5-10 s and rinsing for 1-3 min; putting tissues in phosphate-buffered saline to return to blue and rinsing for 1-3 min; putting tissues in 85% ethylene alcohol for 3-5 min; staining with eosin for 3-5 min and rinsing for 3-5 s; dehydrating with 80, 90, 95, and 100% graded alcohol; vitrification by dimethylbenzene
; and sealing with neutral balsam.
After the paraffin sections were dewaxed in dimethylbenzene
and rehydrated through a graded series of alcohol, antigen retrieval was performed with citric acid-hydrogen phosphate two sodium buffers.
After the nuclei were colored dark brown, the sections were stained by hematoxylin, dehydrated by ethanol, vitrificated by dimethylbenzene
, and mounted by neutral resins.
Brain tissue slices were rinsed in 0.1 M PB and incubated with horseradish peroxidase-labelled goat anti-rabbit IgG (ZHGB, 1:200) at 37[degrees]C for 30 min, followed by rinsing, DAB staining, air-drying, vitrification with dimethylbenzene
, and sealing with neutral gum.
Ten sections (100 [micro]m apart) from each mouse brain were selected and deparaffinized by alcohol and dimethylbenzene
. For DNA denaturation, the sections were incubated in citric acid antigen retrieval buffer (pH = 6.0) at 95[degrees]C for 10 min.
Finally the sections were stained by the DAB (boster) and hematoxylin, followed by dehydration and transparency in a graded series of alcohol and dimethylbenzene
As a routine procedure, the cells were developed for about 5 min before the reaction was terminated and were fully washed using running water, counterstained with hematoxylin, differentiated using 0.1% hydrochloric-alcohol solution, and washed using running water, followed by dehydration with gradient ethanol, vitrification with dimethylbenzene
, and mounting with neutral balsam, and the images were recorded.
The other three age estimation structures (namely vertebrae, opercular bones and cleithra) were immersed in dimethylbenzene
to become transparent, and then photographed and saved.
The PBS mixture was vortexed with dimethylbenzene
for 60 s and incubated at 37[degrees]C for phase separation.