Samples were centrifuged at room temperature for 15 min at 1509.3 g Plasma aliquots were separated in Eppendorf microtubes containing inhibitors of kinin degradation--orthophenanthroline, ethylene-diamine tetraacetic acid (EDTA), dipyridyl
, and sodium tetrathionate--and immediately stored at -20[degrees]C.
For Fe screening, Perl's Prussian Blue (PPB) and 2,2' dipyridyl
stain have been reported, with Zn screening achieved by staining with Dithizone (DTZ, diphenyl-thio-carbazone) and Zincon[R] (2-carboxy-2-hydroxy-5-sulfoformazyl benzene).
In other three clean stoppered tubes, 0.5 mL of each xylene layer was transferred, to this 0.5 mL of dipyridyl
reagent was added.
The concentration of dipyridyl
capable of inducing observable expression of iron-uptake OMPs without completely inhibiting growth was determined by inoculating the colonies in 5 mL BHI broth containing 0, 50,100,150, 200, 250, 300, 350, and 400 [micro]M of 2.2'-dipyridyl.
(1973) which involves the conversion of ferric ions to ferrous ions by a-tocopherol and the formation of red coloured complex with 2, 2 dipyridyl
measured at 520nm.
The clinical isolates were grown overnight in M9 broth containing the iron chelator 2-2' dipyridyl
. Strains were spotted onto hardened dipyridyl
minimal agar plates.
DESIGN, SYNTHESIS AND COMPLEXATION CHARACTERISTICS OF A SIMPLE DIPYRIDYL
Colorimetric iron determination with dipyridyl
showed that the ferritin contained <10 Fe atoms per molecule.
Brocklehurst, "A convenient method of preparation of high activity urease from Canavalia ensiformis by covalent chromatography and an investigation of its thiol groups with 2, 2; dipyridyl
disulphide as a thiol titrant and reactivity probe, " Biochemical Journal, vol.
These ions formed a red complex with [varies]-[varies] Dipyridyl
reagent and it was measured as the color range at 520 nm.