Gel foam and Dulbecco
's modified Eagle's medium were supplied by Life Technologies (India) Pvt.
Between 2001 and 2008, he worked as an associate telethon scientist at the Epigenetics and Genome Reprogramming Lab of the Dulbecco
Avant que Madame Rahma Bourquia, Directrice de l'INE et Philippe Dulbecco
, Inspecteur General IGAENR, MEN et MESRI (France) ne viennent, tour a tour, presenter a l'assistance les resultats du projet de jumelage entre l'INE et le CIEP, la parole a ete donnee a Monsieur RaphaE1/2l Martin de Lagarde, Ministre Conseiller a l'Ambassade de France au Maroc qui s'est rejoui du renforcement des capacites d'expertise de l'Instance Nationale d'Evaluation et de la dynamique mise en oeuvre par cette derniere.
Twenty-four hours before transfection, 293T cells were seeded into 6-cm dishes (1 x 105 cells/dish) in Dulbecco
's modified Eagle's medium (DMEM; Gibco, CA, USA) containing 10% fetal calf serum (Gibco-Invitrogen, Grand Island, NY, USA) and 1% penicillin/streptomycin and maintained at 37[degrees]C in a 5% CO[sub]2 atmosphere.
For example, a comparison of the most widely used "classical" synthetic media, Dulbecco
's Modified Eagle Medium (DMEM) and Roswell Park Memorial Institute 1640 (RPMI1640), to normal human or mouse plasma revealed significant disparities in glucose, micronutrient, and electrolyte concentrations (Arigony et al., 2013; McKee and Komarova, 2017).
's modified Eagle's Medium (DMEM), fetal bovine serum (FBS), penicillin/streptomycin and trypsin 0.25% were purchased from Hyclone (GE Healthcare Life Science, Pittsburgh, PA).
In Austria, we eluted the samples from the ViveST with 1 mL of Dulbecco
's modified Eagle medium high-glucose medium and stored them at -80[degrees]C.
Cells were cultured in Dulbecco
's modified Eagle's medium (DMEM)/F12 medium (Cellgro, Manassas, VA, USA) supplemented with 5% horse serum (Lonza, Allendale, NJ, USA), insulin (10 mg/ml; Sigma-Aldrich, St.
Two days post confluence, cell differentiation was initiated by incubation with 2 [micro]g/ml insulin, 0.25 dexamethasone, and 0.5 mM isobutyl-methylxanthine (IBMX) in Dulbecco
's modified eagle media (DMEM) containing 10% fetal bovine serum and 1% antibiotic antimycotic for 48 h.
MCF-7 cells were cultured in 5% C[O.sub.2] atmosphere at 37[degrees]C in Iscove's Modified Dulbecco
's medium containing 10% FBS and 1% antibiotic (penicillin-streptomycin).
The cell pellet containing ADSCs was collected and washed with fresh Dulbecco
's Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS; Gibco-Invitrogen, Carlsbad, CA, USA) to remove residual enzyme after the digestion, and the digested cells were incubated in a T25 flask (illustration of the procedure is shown in Figure 1).