Previous studies have demonstrated a significant reduction in the expression of cell-to-cell and cell-to-matrix adhesion molecules, such as E-cadherin
(E-CAD) in the metastatic PCa cells.
Integrin and E-cadherin
and MMPs namely ICAM (Intracellular adhesion molecules) have been discovered in cells of menstrual effluent, endometrium, peritoneal fluid, peritoneum, and endometriotic cells (13).
In the present study, we found for the first time that Baohuoside I (BHS-1), a natural flavonol glycoside inhibits BaP-induced A549 lung cancer metastasis by modulating the expressions of p-STAT3, COX-2, MMP-2, MMP-9, E-cadherin
Labelling the Cyclins and E-cadherin
Proteins by Immunohistochemical Analyses
The plasticity of a cancer cell depends crucially on the presence of a cell adhesion molecule: the protein E-cadherin
. Located on the cell surface, it is responsible for cell-to-cell contacts.
In the tissues of diferent groups, the level of E-cadherin
was semi-quantitatively determined (absent (0), slight (1): up to 20% positive; moderate (2): 21%-50% positive; potent (3): 51%-100%).
During Western blot analysis, rabbit monoclonal antibodies specific to human were employed as primary antibodies including ROCK-1 (ab97592, Abcam, Cambridge, MA, USA) (1:1000), [sz]-catenin (ab32572, Abcam) (1:5000), cyclin D1 (ab134175, Abcam) (1:10,000), E-cadherin
(ab1416, Abcam) (1:50), N-cadherin (ab18203, Abcam) (1:1000), and vimentin (ab92547, Abcam) (1:1000).
Currently, there are few reports in the literature regarding the role of cell adhesion proteins, such as E-cadherin
(ECAD), and neural cell adhesion molecule (NCAM) in pituitary adenomas, as well as of SLUG, which is an important transcriptional regulator of ECAD.
To test this, the expression of the epithelial marker E-cadherin
and the mesenchymal marker vimentin was analyzed using QRT-PCR and Western blotting assays.