15 phytochemicals including 5 fatty acids, methyl iso-pentadecanoate (37), methyl pentadecylate (40), isopropyl myristate (41), methyl elaidate
(56), and 9Z,12E-Octadecadienoic acid ME (60); 2 fatty aldehydes, nonanal (16) and 2E-decenal (20); 2 fatty alcohols, lignocerol (68) and pentacosyl alcohol (73); 4 isoprenoids, phytane (36), iso-phytol (42), hexahydrofarnesyl acetone (43), and E-phytol (57), and 2 phytosteroids, O-Sitosterol (79) and fucosterol (81) were identified for the first time from S.
Therefore, to quantify trans fatty acid was taken as parameter the elaidate
fatty acid "C18:1t" (purity [greater than or equal to] 99%, Sigma-Aldrich).
Para isso, foi utilizado padroes da Sigma Aldrich - USA: E4762 Methyl elaidate
, L2251 Methyl linoelaidate, V1381 trans-vaccenic acid methyl ester, O4754 Oleic acid methyl ester e Supelco 37 de mistura Fame 1X1ml CH2CL2.
Elysian, MN, USA) and included mixture RL-461 and FAME of the following fatty acids: myristelaidate (C14:1-t9), palmitelaidate (C16:1-t9), petroselaidate (18:1-t6), elaidate
(C18:1-t9), vaccenate (C18:1-t11), conjugated linoleate (CLA), linoelaidate (C18:2-t9,t12), and eicosenoate (C20:1-t11).