For laboratories that need to universally transfect or transform all cell types of organisms, instruments that offer both square and exponential decay waveforms such as the BTX Gemini Twin Wave Electroporator
offer both square and exponential decay modes for universal electroporation of any sample.
coli DH10[alpha] competent cells and then electroporated with electroporator
(Eppendorf EporatorA(r), Hamburg, Germany) set at 2.4 kV using cuvette with 2mm gap width (Yanagisawa et al., 2004).
After injection, paddle-type electrodes were placed on either side of the head of the embryo, and five 60 ms square pulses with 600 ms intervals at 35 V were applied with an electroporator
(NAPA gene, CUY21, Japan).
Commercial, GMP-compliant electroporation equipment is now also available --MaxCyte is developing a number of clinical-grade platforms that can support GMP-compliant electroporation of cells at high efficiencies, while Miltenyi has developed a closed-system electroporator
as part of their CliniMACS line of equipment.
Electrochemotherapy treatments were performed using an electroporator
device developed by the Department of Electric Engineering of Engineering School of Universidade Federal de Minas Gerais.
Tolvaisiene, "Microsecond electroporator
optimization for parasitic load handling and damping", Elektronika ir Elektrotechnika, vol.
Electric pulses generated by an electroporator
ELECTRO cell B10 HVLV (BETA tech, Saint-Orens-de-Gameville, France) were delivered 10 min after injection of plasmid DNA through two parallel stainless steel electrodes with a 6 mm distance between them.
SV40 large T antigen-transfected human leukemic Jurkat T cells (Jurkat-TAg) in logarithmic growth phase were transfected with plasmid DNAs by electroporation using NEPA21 Super Electroporator
coli ElectroMAX DH5a competent cells were transformed with the extracted plasmids DNA by electroporation (Micro-Pulser electroporator
; Bio-Rad, USA).
Two microliters of the linear transposable element-EZ Transposase were mixed with 25 [micro]l of the electrocompetent cells and poured into an electroporator
cell (0.1 cm) and the electrotransformation was fulfilled (2.5 Kv, 200 W and 25 mF).
Cells were electroporated in a 4 mm cuvette in an EPI2500 electroporator
(Fischer, Heidelberg, Germany) at 370 V for 10 ms.
MG-63 and MG-63/DOX cells were transfected using Opti-MEM (Life Technologies, Grand Island, NY) containing 10 [micro]g plasmids and Super Electroporator
NEPA21 system (NEPAGENE, Japan).