Similarly maximal production of endoglucanase
The identification of endo 1, 6 beta D- glucanase, endoglucanase
D and endoglucanase
E putatively responsible for its cellulolytic activity, the cloning and the recombinant expression of the gene in Escherichia coli, was characterized revealing to exhibit a relevant clear halo zone formation around the colonies on phytagel plates.
Synthesis of endoglucanase
gene and construction of expression vector
Cellulase and hemicellulase specific activities were measured by the dinitrosalicylic acid (DNS) method using solutions of 1.5% carboxymethyl cellulose (endoglucanase
), 2% birchwood xylan (xylanase), and 0.5% locust bean gum (mannanase) .
According to the reports, the monocomponent endoglucanase
seems to give a better pretreatment effect [170,199,200].
cel12a (ID 123232) was the second most upregulated gene in this condition, followed by a [beta]-mannanase.
presented a green pretreatment approach with exoglucanase and endoglucanase
enzymes to produce NFC with controlled length for special applications.
activity (CMCase) was determined according to the method described by Ghose (1987) using a reaction mixture containing 0.5 mL of the enzyme solution with 0.5 mL of 2% carboxymethylcellulose solution in citrate buffer (0.05M, pH 4.8), which was incubated at 50[degrees]C for 30 minutes.
The efficient hydrolysis of cellulosic fraction of plant cell wall requires the synergistic action of the cellulolytic enzymes-exoglucanase, endoglucanase
, cellobiohydrolase and [beta]-glucosidase, and some hemicellulolytic enzymes [4, 5].
GH6 family includes both endoglucanases
and cellobiohydrolases, many GH6 endoglucanases
have been reported, such as Thermobifida fusca Cel6A (Ali et al., 2015), Thermobifida halotolerans GH6 endoglucanase
(Yin et al., 2015), and Cellulosimicrobium funkei CelL (Kim et al., 2016).
Both were prepared by overnight incubation of SCH-PK and SCH with endoglucanase
from Penicillium funiculosum (kindly provided by Erbsloh, Geisenheim, Germany) at 50[degrees]C.
 proposed that the inhibition of ethylene production by UV-C treatment maybe responsible for maintaining lower expression levels of genes coding for cell wall degrading enzymes which are known to be activated by this hormone, such as pectin methylesterase, polygalacturonase, and endoglucanase