patents - US 9,855,297 and US 9,890,393 - for the invention of certain uses of RNA-guided endonucleases
, such as Cas9 or Cpf1, for the genetic engineering of T-cells.
Their results confirm the high specificity of CRISPR endonucleases
and demonstrate that sequence capture can be used as a high-throughput genome-wide approach to identify off-target activity.
Clustered regularly interspaced palindromic repeat (CRISPR)-associated genes (CRISPR-Cas) have emerged as a novel class of sequence-specific endonucleases
with unparalleled flexibility, cost-effectiveness and ease of application.
Efficient nonmeiotic allele introgression in livestock using custom endonucleases
The cutting sites of other endonucleases
(Hinc II, Acc I, and PflM I) were not affected by the existing mutations.
This DNase is more active on double-stranded DNA than on single-stranded DNA and differs from the other three types of endonucleases
that are involved in DNA fragmentation (DNase I, DNase II, and NUC 18) .
Then 12 microliters of the amplified fragment (range 173-208 bp depending on HPV type) were used in two different restriction reactions with endonucleases
Rsal and Tru91, respectively, and analyzed using a capillary electrophoresis system.
Those genes make endonucleases
--DNA-cutting enzymes that target one spot in an organism's entire genetic catalog, or genome.
This event triggers the release of pro-apoptotic proteins, including cytochrome C, from the mitochondrial intermembrane space to the cytosol where they activate caspases and endonucleases
that initiates apoptosis.
Through the combination of CRISPRs and RNA-guided endonucleases
, such as Cas9, ("Cas" stands for CRISPR-associated), bacteria are able to utilize small customized crRNA molecules (for CRISPR RNA) to guide the targeting and degradation of matching DNA sequences in invading viruses and plasmids to prevent them from replicating.
Results: Recombinant plasmid pcDNA3--HBX was confirmed by restriction endonucleases
digestion and colony-PCR.
Para a identificacao e confirmacao da especie, foram realizadas duas analises de restricao (RFLP) do amplicon de 890bp (S2), obtidas atraves de digestoes com as endonucleases
XmnI e CfoI com incubacao em banho-maria a 37[degrees]C por 2h, segundo metodologia proposta por PAILLARD et al.