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 (ĕn′dō-no͞o′klē-ās′, -āz′, -nyo͞o′-)
Any of a group of enzymes that catalyze the hydrolysis of bonds between nucleotides in the interior of a DNA or RNA molecule.


(Biochemistry) an enzyme that is responsible for scission of a nucleic acid chain, the action of which is not confined to the terminal nucleotide. Compare exonuclease


(ˌɛn doʊˈnu kliˌeɪs, -ˌeɪz, -ˈnyu-)

any of a group of enzymes that degrade DNA or RNA molecules by breaking linkages within the polynucleotide chains.
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.endonuclease - a nuclease that cleaves nucleic acids at interior bonds and so produces fragments of various sizes
nuclease - general term for enzymes that catalyze the hydrolysis of nucleic acid by cleaving chains of nucleotides into smaller units
restriction endonuclease, restriction enzyme, restriction nuclease - any of the enzymes that cut nucleic acid at specific restriction sites and produce restriction fragments; obtained from bacteria (where they cripple viral invaders); used in recombinant DNA technology
References in periodicals archive ?
Elements in abasic site recognition by the major human and Escherichia coli apurinic/apyrimidinic endonucleases Nucleic Acids Res 26:2771-2778.
The pullulan DNA was ligated both forward (sense) and backward (antisense) into the pYES2 through the use of several restriction endonucleases.
Demand for DNA polymerases, restriction endonucleases and other workhorse laboratory enzymes will grow steadily; innovative products may also spur demand.
1) Coupling endonucleases with DNA end-processing enzymes to drive gene disruption, Nature Methods doi:10.
Cellectis develops and produces engineered site-specific endonucleases such as Meganucleases and TALENTM which have recently emerged as the most powerful approach in Genome engineering.
PCR products were digested with different restriction endonucleases (DdeI, MboI, MboII, and MseI.
In this case, methylation analysis involving the use of methylation-sensitive restriction endonucleases could be used, thus cutting hypomethylated RASSF1A from maternal blood cells and leaving the hypommethylated placental RASSF1A intact for amplification.
coli O157 clusters with two or more restriction endonucleases is necessary before public health resources are allocated to follow-up investigations.
Degradation of internucleosomal (linker) DNA segments as a consequence of activation of endogenous endonucleases is considered a characteristic end point of apoptosis and is analyzed using gel electrophoresis.
Functionally, these deoxyribozymes are cousins of protein enzymes known as restriction endonucleases.
Precision BioSciences has already produced hundreds of custom endonucleases for partners and internal development that can precisely alter naturally occurring sequences within genomes.