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 (ĕn′dō-no͞o′klē-ās′, -āz′, -nyo͞o′-)
Any of a group of enzymes that catalyze the hydrolysis of bonds between nucleotides in the interior of a DNA or RNA molecule.


(Biochemistry) an enzyme that is responsible for scission of a nucleic acid chain, the action of which is not confined to the terminal nucleotide. Compare exonuclease


(ˌɛn doʊˈnu kliˌeɪs, -ˌeɪz, -ˈnyu-)

any of a group of enzymes that degrade DNA or RNA molecules by breaking linkages within the polynucleotide chains.
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.endonuclease - a nuclease that cleaves nucleic acids at interior bonds and so produces fragments of various sizes
nuclease - general term for enzymes that catalyze the hydrolysis of nucleic acid by cleaving chains of nucleotides into smaller units
restriction endonuclease, restriction enzyme, restriction nuclease - any of the enzymes that cut nucleic acid at specific restriction sites and produce restriction fragments; obtained from bacteria (where they cripple viral invaders); used in recombinant DNA technology
References in periodicals archive ?
patents - US 9,855,297 and US 9,890,393 - for the invention of certain uses of RNA-guided endonucleases, such as Cas9 or Cpf1, for the genetic engineering of T-cells.
Their results confirm the high specificity of CRISPR endonucleases and demonstrate that sequence capture can be used as a high-throughput genome-wide approach to identify off-target activity.
Clustered regularly interspaced palindromic repeat (CRISPR)-associated genes (CRISPR-Cas) have emerged as a novel class of sequence-specific endonucleases with unparalleled flexibility, cost-effectiveness and ease of application.
The cutting sites of other endonucleases (Hinc II, Acc I, and PflM I) were not affected by the existing mutations.
This DNase is more active on double-stranded DNA than on single-stranded DNA and differs from the other three types of endonucleases that are involved in DNA fragmentation (DNase I, DNase II, and NUC 18) [18].
Then 12 microliters of the amplified fragment (range 173-208 bp depending on HPV type) were used in two different restriction reactions with endonucleases Rsal and Tru91, respectively, and analyzed using a capillary electrophoresis system.
Those genes make endonucleases --DNA-cutting enzymes that target one spot in an organism's entire genetic catalog, or genome.
This event triggers the release of pro-apoptotic proteins, including cytochrome C, from the mitochondrial intermembrane space to the cytosol where they activate caspases and endonucleases that initiates apoptosis.
Through the combination of CRISPRs and RNA-guided endonucleases, such as Cas9, ("Cas" stands for CRISPR-associated), bacteria are able to utilize small customized crRNA molecules (for CRISPR RNA) to guide the targeting and degradation of matching DNA sequences in invading viruses and plasmids to prevent them from replicating.
Results: Recombinant plasmid pcDNA3--HBX was confirmed by restriction endonucleases digestion and colony-PCR.
Para a identificacao e confirmacao da especie, foram realizadas duas analises de restricao (RFLP) do amplicon de 890bp (S2), obtidas atraves de digestoes com as endonucleases XmnI e CfoI com incubacao em banho-maria a 37[degrees]C por 2h, segundo metodologia proposta por PAILLARD et al.