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Related to Esculin: Aesculin
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Suspected organisms were identified by conventional biochemical tests described earlier.18 Identification tests included citrate utilization test, urease test, indole test, catalase test, DNAse test, Mannitol fermentation test, Esculin hydrolysis test and inoculation of KIA media to test for sugar fermentation and H2S.
Streptococci were initially identified using CAMP test, esculin hydrolysis, hemolysis, and no growth on MacConkey agar.
Whereas gram-negative microbes were identified by oxidase, bile esculin hydrolysis and analytical profile index (API) 20E.
The isolates were identified based on Colony characteristics, Gram staining, Motility and Conventional biochemical test like oxidase test, catalase test, indole, methyl red, Voges-Proskauer, citrate utilization, urease production oxidative fermentative test (Hugh-Leifson medium) for glucose, utilization of 10% lactose, gelatin liquefaction, lysine and ornithine decarboxylation, arginine dihydrolase test, growth at 42[degrees]C and 44[degrees]C, esculin hydrolysis and ONPG test.
It is now considered unsafe by the FDA because it contains a number of toxins such as quercitin, rutin, shikimic acid and the very dangerous esculin. Honey gathered from Horse Chestnut pollen is poisonous.
haemolytica is characterized by hemolysis in blood agar, growth on MacConkey's agar and biochemical positivity and negativity for beta-galactosidase and esculin hydrolysis tests, respectively (MARKEY et al., 2013).
Isolates were identified based on black colonies on bile esculin agar media, Gram staining, catalase test and bacterial growth in 6.5% NaCl.
The purified isolates were then subjected to presumptive identification tests, including Gram staining, catalase and bile esculin, growth at 45[degrees]C, and 6.5% of NaCl.
Catalase tests, growth at 6.5% salt, bile esculin hydrolysis, and the pyrrolidonyl arylamidase (PYR) test were used to identify the genera and strains.
For the routine culture, the aspirate was plated to sheep blood agar, chocolate agar, MacConkey agar, mannitol salt agar and esculin agar, along with a thioglycollate broth.
Survivors were enumerated by serial dilutions of the cell suspensions and by plating out on Tryptic soy agar containing yeast extract, esculin and ferric ammonium citrate (TSAYE-EF).