Extraction and estimation of Alginate For extraction of exopolysaccharide
, 1 mL of (0.5M) EDTA sodium salt solution and 0.5 mL of (5.0M) NaCl solution was added to the fermentation media.
Treatment of biofilm-associated persistent infections is an emerging issue for clinicians as bacterial cells adhere with human epithelial cells or indwelling medical devices such as implants and catheters used in urinary tract and respiratory infections.1 Biofilms are complex surface-associated communities where bacterial cells are enclosed by self-produced extra cellular polymeric substances (EPS), which mainly consist of exopolysaccharides
, proteins and extracellular deoxyribonucleic acid (DNA).2-4 Pseudomonas (P.) aeruginosa is an important human pathogen which causes many infections ranging from wound infections to cystic fibrosis.
is produced by microalgae of the genus Parachlorella.
A cellulosic exopolysaccharide
produced from sugarcane molasses by a Zoogloea sp.
It is the matrix that forms biofilms, which components include bacterial cells, exopolysaccharides
, proteins, nucleic acids, glycoproteins, phospholipids, debris, and inorganic matter (SUTHERLAND, 2001).
Extraction and Quantification of Exopolysaccharide
Tian et al., "Molecular characteristics of an exopolysaccharide
from Lactobacillus rhamnosus KF5 in solution," International Journal of Biological Macromolecules, vol.
Several studies have suggested the organism's higher affinity for endocardium due to the production of a significant amount of exopolysaccharide
and propensity to bind with fibronectin in the extracellular matrix, which further expands their virulence [4, 6].
aureus were established and then exposed to different bacteriophage-derived proteins that catalyze degradation of the key biofilm extracellular polymers peptidoglycan or exopolysaccharide
These bacteria encase themselves in a proteinaceous matrix called exopolysaccharide