To synthesize first strand cDNA, DNasetreated RNA (0.5 y.g) was used with an Exscript
RT reagent kit (Takara, Tokyo, Japan).
With a standard protocol provided by the manufacturer, qRT-PCR was performed in the ABI PRISM 7300 Fast Real-Time PCR System (Ambion, USA) using the SYBR ExScript
qRT-PCR Kit (Takara, China), under the following conditions: 95[degrees]C for 5 min, 95[degrees]C for 10 s, and 40 cycles at 60[degrees]C for 30 s.
Real time RTq-PCR was performed with SYBER ExScript
RT PCR Kit (TaKaRa, Inc., Japan) to determine the expression level of Catalase (Cat2) gene.
Total RNA was extracted from liver samples and HepG2 cells of each group and treated by ExScript
RT reagent kit (TAKARA, Kusatsu, Japan) for reverse transcription (RT).
First-strand cDNA was synthesized from 1mg total RNA using an ExScript
RT reagent kit (Takara Bio Inc., Shiga, Japan) following the operation manual.
The expression was detected in an Eppendorf Mastercycler (Brinkman Instruments, Westbury, NY) using the SYBR ExScript
RT-PCR Kit (Takara, China).
We generated cDNA from 500 ng DNase-treated RNA using ExScript
RT-PCR Kit (Takara Co.
RT reagent kit and SYBR Premix Ex Taq (perfect real time) were obtained from TaKaRa (DaLian, China).
We generated cDNA from 500 ng DNase treated RNA using ExScript
RT-PCR Kit (Dalian Takara Co.