In both LIF-treated (Vitrified and non-vitrified) groups, the expression ratio of pro-apoptotic genes (Fas, FasL
, caspase 3 and 8, and p53) was lower and the expression of anti-apoptotic gene (BIRC5) was higher than non-LIF-treated groups (p<0.05).
protein is a key active ingredient in Cellect's product line and technology for cell separation and functional selection of stem cells.
Let-7g-5p HMGA2  miR-10b-5p Bcl6  miR-19a Undetected  miR-19b Smad7  miR-21 FasL
, PDCD4 [41, 42] miR-22-3p HDAC6  miR-23a Fas, Runx2 [70, 71] miR-24 Tcf-1  miR-27 APC  miR-29a-3p DKK1, Kremen2, sFRP2  miR-30 family Smad1, Runx2, LRP6  miR-100 BMPR2  miR-124 Dlx5, Dlx3, Dlx2  NFATc1, RhoA, Rac1  miR-125 ErbB2  miR-140-5p BMP2  miR-142 APC  miR-148a Kdm6b  MAFB  miR-2861 HDAC5  Table 3: miRNA dysregulated in plasma of osteoporotic patients and their function.
AREG and FasL
concentrations in supernatants from monocyte cultures were determined by enzyme-linked immunosorbent assay (ELISA).
In that study, the increased level of granulysin in blister fluids from the skin lesions of SJS/TEN patients was much higher than the levels of other cytotoxic proteins, such as perforin, granzyme B, and FasL
, and depleting the granulysin reduced the cytotoxicity .
MSCs Reduced Infiltration of CTLs in the Lungs of LLC1-Treated Mice and Attenuated Expression of FasL
, Perforin, and CD107 on Their Surface.
The expression levels of apoptosome containing heme oxygenase 1 (HO-1), apoptosis protease-activating factor-1 (APAF-1), caspase-3, cleaved-caspase-3, B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax), poly ADP ribose polymerase (PARP), cytochrome c, factor-associated suicide (FAS), Fas ligand (FASL
), caspase-8, and NOX2 were assessed by Western blotting.
Then the membranes were washed in TBS/T and the primary antibodies like caspase 3 (1: 1000 dilution, Cell Signaling Technology, CST, USA), cleaved caspase 3 (1: 1000 dilution, CST), caspase 7 (CST), TNF-a (1: 2000 dilution, Abcam), IL-1[beta] (1:1000 dilution, Abcam), BCL-2 (1: 1000 dilution, CST), BCL-xL (1: 1000 dilution, CST), FasL
(1:1000 dilution, Santa Cruz), F4/80 (a mouse macrophage biomarker, 1:1000, Abcam), stat3 (1:1000 dilution, CST), phosphorylated-stat3 (Tyr705,1: 1000 dilution, CST), p44/42 MAPK (Erk1/2) (1:1000 dilution, CST), Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204, 1:1000 dilution, CST), F4/80 (1: 1000, Abcam, USA), Ly6G (1: 1000, Invitrogen, Life Technology), and [beta]-actin (1: 1000 dilution, Santa Cruz) were added and incubated at overnight in 4[degrees]C.
determined that FasL
is a functional target of miR-21; they stated that 37-UTR region of the FasL
mRNA contains conserved miR-21 binding sites, and mutation of these sites canceled miR-21 mediated downregulation of FasL
In addition, trifolin increased the levels of the death receptor involving the Fas/Fas ligand (FasL
) and Fas-associated protein with the death domain (FADD), which consequently activated caspase-8, caspase-9, caspase-3, and the proteolytic cleavage of poly (ADP-ribose) polymerase (PARP).
Hamid Farahi, the head of the team dispatched to the site by the Iranian Cultural Heritage and Tourism Organization (ICHTO), said the skeleton was discovered six meters (20 feet) below the surface in Sar Sakhti-ye Olya village in Shazand, and was transferred to Chahar Fasl
(Four Seasons) Museum in Arak.
To explore the underlying mechanism, they compared the expression levels of Fas in tumor cells and FasL
in TILs in IMPC and MC and found both Fas and FasL
expression levels were significantly less in IMPC than in MC.