This variable interference further limits the utility of FPIA
after [CPDG.sub.2] administration.
We evaluated potential interference of Asian, American, and Indian ginsengs on serum digoxin measurement both in vitro and in vivo in a mouse model with the ECLIA-digoxin assay and a turbidimetric assay (TIA) on Bayer's ADVIA Chemistry systems and compared the results with the results obtained by using FPIA
. To our knowledge, the potential interference of Asian, American, and Indian ginsengs with the ECLIA or the turbidimetric digoxin assays has never been studied.
1 presents the geometric means of plasma Hcy obtained by HPLC and FPIA
and plasma SAM and SAH after blood sample incubation over a period of 0, 2, 6, and 24 h at room temperature or at 4 [degrees]C.
. A fully automated fluorescence polarization immunoassay system is described and applied to therapeutic substances.
(19-20) Hemolysis, icterus, and turbidity from lipids are common sources of assay interference, and most kit manufacturers list them in the kit insert; however, conjugated bilirubin in serum specimens was reported to interfere in a fluorescence polarization immunoassay (FPIA
) for vancomycin.
Mean (95% CI) (a) Assay Mean (SD) Hcy, difference, method Anticoagulant [micro]mol/L [micro]mol/L HPLC(a) EDTA 10.9 (5.1) -1.8 ( 2.1 to 1.6) Acidic citrate 12.8 (5.5) HPLC(b) EDTA 14.1 (5.0) 2.8 (2.5-3.1) Acidic citrate 11.3 (4.7) FPIA
EDTA 12.1 (5.5) -0.1 ( 0.3 to 0.0) Acidic citrate 12.3 (5.4) Regression Assay coefficient Mean proportional method (95% CI) bias, (b)% HPLC(a) 1.01 (0.96-1.07) -16 HPLC(b) 0.75 (0.72-0.78) 24 FPIA
0.95 (0.92-0.98) 2 Assay Lower limit of Upper limit of method agreement, % agreement, % HPLC(a) -40 15 HPLC(b) -4 62 FPIA
-19 16 (a) CI, confidence interval; FPIA
, fluorescence polarization immunoassay.
Louis, MO), Beckman enzyme immunoassay (EIA) reagents on a Synchron[R] CX-9 (Children s Mercy Hospital, Kansas City, MO), or fluorescence polarization immunoassay (FPIA
) technology on an Abbott Ax5YN1[R] (Children s Hospital of Wisconsin, Milwaukee, WI) according to the manufacturer's instructions in each case.
Although the fluorescence polarization immunoassay (FPIA
) is less subject to interference from adulterants when compared to the EMIT assay, some interferences have been reported.
The fluorescence polarization immunoassay (FPIA
), run on Abbott's IMx and AxSYM platforms (12, 26), is widely used in both research and routine laboratory settings.
In the clinical trial conducted with cardiac transplant recipients, whole-blood CsA was measured with a fluorescence polarization immunoassay (FPIA
; Abbott TDx).
Our search narrowed the field to three instruments, each with specific technological advantages of its own: 1) a dedicated fluorescence polarization immunoassay (FPIA
) batch analyzer, 2) a random access multitest analyzer, and 3) a wet chemistry/TDM/toxicology batch analyzer.
For carbamazepine, the intralaboratory variance of the Syva Emit was significantly greater than that for the two Abbott methods, whereas the interlaboratory variance of the CEDIA method was significantly greater than that for the Abbott AxSYM, Roche fluorescence polarization immunoassay (FPIA
), and HPLC.