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Immunophenotyping: To phenotype cell-surface an- tigens fourth-passage cells were stained with fluo- rescein isothiocyannate (FITC) or phycoetrythrin (PE)-conjugated antibodies specific for the follow- ing human antigens CD90-FITC CD133-PE CD44- FITC CD34- FITC and CD105-FITC (BioLegend USA).
Moreover, no study concerned about the effect of aging and thermocycling on the bond strength of contemporary adhesive to the fluo- rosed enamel of patients in endemic areas like Saudi Arabia.
One of such optical technologies is called Autofluorescence or Tissue fluo- rescence imaging" commercially available by the brand name VELscope".
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