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The most widely used diagnostic tests for tear film examination are tear break-up time (TBUT) test, corneal and conjunctival staining with fluorescein, rose bengal and lissamine green dye, Schirmer test, tear film osmolarity test and impression cytology.
Tear break-up time fluorescein test is widely accepted in dry eye diagnosis and is considered more reliable than Schirmer test as it can be repeated (13), and is minimally invasive.
Conjunctival and corneal epithelial erosions are stained with fluorescein dye (fluorescein invades the sites of disruption between cell junctions).

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