For this study a non-drug therapy, the autohemotransfusion, was proposed in order to alleviate the inflammatory process in rats induced by Complete Freund's Adjuvant
Intriguingly, NLRP3 inflammasome is activated in the skin tissue by complete Freund's adjuvant
(CFA) injection in rats (9).
With Freund's adjuvant
, the highest absorbance was 7,044[+ or -]0,630; with the Montanide[TM] adjuvants including IMS 1313 VG NPR, ISA 201 VG and ISA 61 VG; 4.881[+ or -]0.318, 5.283[+ or -]0.466, and 9.848[+ or -]0.630 absorbances were obtained respectively after the last immunization.
Immunized group mice were immunized subcutaneously with 100 [micro]L of antigen-adjuvant suspension containing 20 [micro]g peptide vaccine emulsified in complete Freund's adjuvant
with 0.1 ml of complete Freund's adjuvant
(CFA) containing Mycobacterium tuberculosis (H37Ra) (Fisher Scientific) and emulsified with an equal amount of phosphate buffered saline in some experiments.
The platelet suspension was mixed with an equal volume of complete Freund's adjuvant
or incomplete Freund's adjuvant
(Sigma, USA) via ultrasound.
, a mixture of mineral oil and killed tuberculosis bacteria, is required.
Twenty-four rats received a single intradermal injection of complete Freund's adjuvant
(CFA; heat-killed Mycobacterium butyricum were well suspended in mineral oil, 10 mg/ml, 25 mg/kg; Difco Lab., Detroit, MI, USA) at the base of tail under deep ether anesthesia.
The peptide vaccines administered with incomplete Freund's adjuvant
(IFA) are ineffective but, when administered with powerful adjuvants such as a toll-like receptor agonist, elicit antitumor CD8 T cell responses .
Arthritis was induced into the rats using Complete Freund's adjuvant
(CFA) into the sub-plantar region of the left hind paw, with treatments commencing orally on day 5 after induction by CFA until 28 days.
First group was immunized with alum adjuvant, second with Freund's complete adjuvant (FCA), third with Incomplete Freund's adjuvant
(IFA) and fourth with Montanide ISA-50 (Incomplete Seppic) adjuvant.
It consists of a suspension of killed HIV-1 virus particles that have been emulsified with Incomplete Freund's Adjuvant
(IFA , a mixture of mannide mono-oleate and a highly purified mineral oil).