Isolated human eosinophils ([10.sup.5]) were preincubated (60 min) with various concentrations of Gencydo[R], Campothecin (100[micro]M) (Tocris, Bristol, UK) or Triton-X100 (0.5%) (Carl Roth, Karlsruhe, Germany) as controls in medium in FCS-precoated wells followed by further incubation for 23 h in presence or absence of 20 ng/ml human GM-CSF
(PeproTech, Hamburg, Germany).
A laboratory-made version of GM-CSF
called Leukine has been used for years to boost the immune systems of certain cancer patients.
concentrations in the supernatants and plasma samples were below ELISA limit of detection.
CSF and GM-CSF
; there are two theories on the mechanism of oral mucositis reduction by G-CSF and GM-CSF
The most significant recent advance in our understanding of PAP has been the identification of a neutralizing autoantibody to GM-CSF
in patients with the idiopathic form of the disease.
IL-17 and IL-17F modulate GM-CSF
production by lung microvascular endothelial cells stimulated with IL-lbeta and/or TNF-alpha.
The researchers confirmed that GM-CSF
mouthwash conferred no benefit to oral mucositis despite promising results in preclinical and phase I trials and further recommended that other agents are similarly scrutinised using an RCT design.
GVAX is made up of lethally irradiated lines of pancreatic cancer cells that were engineered to secrete granulocyte macrophage colony stimulating factor (GM-CSF
The fact that osteoblasts secrete two major cytokines, namely, macrophage colony stimulating factor (M-CSF) and granulocyte-macrophage colony stimulating factor (GM-CSF
) led this group to believe that recombinant BMP-2 may play a major role in hematopoiesis, and further, serve as a potential cytokine in stem cell transplantation.
The 14 women whose tumors expressed the E75 peptide on surfaces of the HER2/neu protein were given a vaccine aimed at that specific target antigen plus granulocyte-macrophage colony-stimulating factor (GM-CSF
) intended to trigger a generalized immune response.
When given to chronically HIV-infected patients during an STI, GM-CSF
blunted viral rebound and substantially prevented decreases in CD4 T cells in the absence of antiretroviral treatment.
5 when a research group led by assistant professor Kenzaburo Tani removed the man's right kidney and then introduced immunity-enhancing GM-CSF
genes into cancer cells taken from the diseased kidney.