Nulliparous stages were classified as: nulliparous 1 (where the germarium
and vitellarium are not differentiated and are without follicles); nulliparous 2 (well-defined ovarioles with multiple follicles, the proximal follicle transparent indicating that it had not completed maturation); and nulliparous 3 (proximal follicle more opaque [mature] and close to ovulation).
Adults resulting from larvae treated with EOs presented fewer regenerative cells in the midguts, which were elongated with a narrower lumen and females presented germarium
and follicular epithelium of the ovarioles with faint nuclei, prejudicing reproduction .
Egg development occurred anteriorly to posteriorly along the ovariole, with two distinctly recognizable regions: the germarium
and the vitellarium.
In the Drosophila germarium
, altered Wingless (Wg; the fly Wnt homologue) signaling occurs in ovarian germline stem cells (GSCs) through removal of its regulators such as dishevelled, armadillo, Axin, and shaggy; this causes GSC loss, influences follicle cell proliferation, and induces differentiation [34, 35].
In the apical region of the follicle (germarium
) numerous cysts were observed just below the germarium
, which is characteristic of the growth zone.
In the present study, this parasite revealed that the position of the gonads, with germarium
anterior superimposed on testis, is consistent with another Ameloblastella species.
(2003) for Pseudonannolene spp., which have unpaired ovaries without pairing of the germarium
, and this is considered by other authors to be the most derived model among the millipedes.
mexicanus Harold, normally complete only three nesting cycles, after which (approximately 180 days following adult emergence) the germarium
of the ovary is very reduced.
In insects treated with 200 ppm azadirachtin, the cyst formation around the spermatogonia in the germarium
was inhibited without any further development to spermatocytes and with the appearance of many vacuoles (Figs 17, 18).
oval, 164-495 (365) in diameter, pretesticular; short uterus; vagina long, with distal papilliform structure (Fig.
The ovarioles were of the telotrophic meroistic type, wherein nurse cells in the distal germarium
provide nutrients to developing follicles contained in the vitellarium (Chapman 1998).