Gram's stain

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Gram stain

 (grăm) also Gram's stain (grămz)
A staining technique used to classify bacteria in which a bacterial specimen is first stained with crystal violet, then treated with an iodine solution, decolorized with alcohol, and counterstained with safranin. Because of differences in cell wall structure, gram-positive bacteria retain the violet stain whereas gram-negative bacteria do not. Also called Gram's method.

[After Hans Christian Joachim Gram (1853-1938), Danish physician.]
American Heritage® Dictionary of the English Language, Fifth Edition. Copyright © 2016 by Houghton Mifflin Harcourt Publishing Company. Published by Houghton Mifflin Harcourt Publishing Company. All rights reserved.
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Noun1.Gram's stain - a staining technique used to classify bacteria; bacteria are stained with gentian violet and then treated with Gram's solution; after being decolorized with alcohol and treated with safranine and washed in water, those that retain the gentian violet are Gram-positive and those that do not retain it are Gram-negative
staining - (histology) the use of a dye to color specimens for microscopic study
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References in periodicals archive ?
Can empiric broad-spectrum antibiotics for ventilator associated pneumonia be narrowed based on Gram's stain results of bronchoalveolar lavage fluid.
From this smear was prepared in a clean glass slide, stained using Gram's stain and viewed under oil immersion power of microscope.
The isolated bacterial colonies on blood agar plates were small, glistening, mucoid, haemolytic and dew drop like, and appeared as gram negative coccobacilli when stained with Gram's stain stain were identified as Pasteurella spp.
We compared polymerase chain reaction (PCR) of cloacal swab samples and fecal Gram's stain (FGS) for diagnosis of active shedding of M ornithogaster in a captive flock of budgerigars (Melopsitiacus undulatus).
The specimen was then transported to the bacteriology laboratory for Gram's stain and possible inoculation to the media.
Direct impression smears were prepared from the biopsy tissues on a clean glass slide and were stained with Gram's stain. Gram's staining shows Gram negative, spiral or S shaped or Seagull shaped organisms with bluntly rounded ends of 0.5-1[micro]m wide and 2.5-5[micro]m long (Fig 1).
Gram's Stain: A drop of uncentrifuged, well mixed urine was taken on a clean grease-free slide and stained by Gram's staining and examined under the oil immersion objective of the microscope (examining 20 fields).
Assessment of lactobacillary flora in wet mount and fresh or delayed Gram's stain. Infect Dis Obstet Gynecol.
Surgical tissue Gram's stain and culture were nonrevealing, but fungal culture grew P.
As DNA tests become more accurate, fewer healthcare professionals are relying on the Gram's stain, which is reliable in only about half of all women, and culture testing, which can delay diagnosis by several days.
Sputum gram's stain in community-acquired pneumococcal pneumonia.
The aspirate was evaluated by Gram's stain, aerobic and anaerobic cultures, and Warthin-Starry silver staining (figure 3).