Shield-stage (~6 hpf
) embryos were exposed to 0, 0.0625, 0.125, 0.25, 0.5, or 1 ng/mL TCDD (311 nM, 95.3% purity; SUPELCO Solutions Within) or vehicle (0.1% DMSO) with gentle rocking for 1 h in 20-mL glass vials (10 embryos/mL).
To examine the development of the nervous system, we collected samples at the following stages: blastula (16 hpf
): early, middle, and late trochophore (18 and 22 hpf
, 28 hpf
, and 32-36 hpf
Samples of larvae for description of embryonic development were taken after fertilization; every 15 min during the first 1 h, every 30 min in the first 13 h, then every hour up to 24 hpf
. Samples of larvae were, thereafter, taken daily through to settlement.
11 of 13 cases had a mitotic index of [less than or equal to] 1 mitosis/50 HPF
, and all cases had a mitotic count [less than or equal to] 4 mitoses/50 HPF
Some authors suggest a value as low as at 6 eosinophils per HPF
, some 15 to 20 [2, 16], some 30 , and some 50 .
Cytological evaluation: All the slides were blindly evaluated by selecting ten high power field (HPF
) to determine the total cellularity of endometrial cells (epithelial cells and PMN) and cellular morphology (Percentage of intact and distorted cells).
Block diagrams of controlled limiter using: a--logarithmic amplifier: b--constant displacement; c--sample-and-hold device; 1--input voltage protection; 2--electronic multiplier; 3--low pass filter (LPF); 4--high pass filter (HPF
); 5--sample-and-hold device; 6--controlled limiter.
The current patient was in the intermediate risk group with 1-4 mitosis per 50 HPF
. Imatinib, a selective protein tyrosine kinase inhibitor, has been demonstrated to be an effective treatment for GISTs and EGISTs.
Newly-hatched larvae (57 hpf
; 28.5[degrees]C) were randomized and exposed in 20 mL glass vials to C-9500A concentration ranging from 0 ppm to 200 ppm (n=6 vials per exposure concentration, 10 larvae per vial), which were produced by serial dilution.
Mitoses are rare, usually fewer than 5 per 10 high-power fields (HPF
) (Figure, A).
Routine blood counts, urine analysis were done for all patients and those showing pus cells >5 per HPF
in centrifuged urine sample were taken as study group and urine culture sensitivity were done.
, the entropy is decreased to 4.6, that means the original image has lost some information because the entropy of original image after grayscale conversion came out to be near 5.