The sperm concentration was estimated using the Haemocytometer
(improved Neubauer's chamber) as described by Salisbury et al.
The concentration of the microconidia suspension was determined with a haemocytometer
and adjusted by dilution to the desired concentration for plant inoculation to obtain a 106 spores/ml.
Conidia in washing suspensions were counted using a haemocytometer
The cells were adjusted to 3 x106 cells/ml with the dilution of normal saline and counting by using haemocytometer
Two indicators of EAC-cell activity were measured in both control and the MTX treated groups: (i) the volume of the fluids inside the peritoneal cavity; (ii) the numbers of EAC cells found in the fluid by using a haemocytometer
Counting was then done using the counting chamber in the haemocytometer
147 Table 5: Number and differences in Nosema spores counted per infested worker bee during the dry and wet seasons: (number of spores = spore counted x (4 x 100) in haemocytometer
slide) Apiary Average no.
The tube was vortexed for 2-3 min to dislodge conidia from the insect and the concentration of conidia was determined using a haemocytometer
The cells were counted in a haemocytometer
at 400X magnification.
Triplicate wells of viable cells were counted using a haemocytometer
and the experiment was repeated three times.
Leucocyte counts: Total leucocytes count (TLC) was done following standard haematological techniques using haemocytometer
The blood cell count before and after incubation of these microspheres with blood was manually counted using a haemocytometer
to study any adhesion of cells onto the microspheres.