A nested polymerase chain reaction (PCR), targeting the mitochondrial cytochrome b gene of Haemoproteus
species, Plasmodium species, and Leucocytozoon species, was performed on frozen tissue samples collected at necropsy.
The visual inspection of the specimens to detect and identify ectoparasite was negative, and only 22.22% (4/18) of the individuals were positive for the presence of hemoparasites, identifying the protozoan Haemoproteus
Evcil ve yabani kanatlilarda Plasmodium, Haemoproteus
, Leucocytozoon ve Trypanosoma soylarina bagli kan protozoonlari gorulmekte ve bu parazitler cesitli kan emen sineklerle nakledilmektedir (1-3).
A new PCR assay for simultaneous studies of Leucocytozoon, Plasmodium, and Haemoproteus
from avian blood.
Ascardia spp and Capillaria spp are the most common nematodes in pigeon (Kirsch and Degenhartdt, 1979) and protozoan infections among Haemoproteus
columbae, transmitted by Pseudolynchia canariensis affects domestic as well as wild pigeon is called as pseudomalaria or pigeon malaria and is fatal to young ones.
Survey for Haemoproteus
spp., Trichomonas gallinae, Chlamydophila psittaci, and Salmonella spp.
spp., Leucocytozoon spp., Plasmodium spp., Trichomonas spp., Eimeria spp., Sarcosystis spp., Babesia spp.
In addition to the direct effects that blood-sucking flies have on hosts due to their bites, louse flies are known vectors of avian pathogens such as Trypanosoma and Haemoproteus
(Baker, 1967; Santiago-Alarcon et al., 2012).
In Pakistan, there is a need of time to develop more and more reliable molecular techniques like PCR in order to improve the diagnostic abilities in laboratories for the diagnosis of vector-borne parasitic infections like Haemoproteus
. This will definitely enhance our ability to intervene against parasitic infections in birds.Key words: Haemoproteus
, Microscopy, PCR, BirdsPrevalence and distribution in birdsThe name of the organism was first coined by Kruse in 1890 when he described a parasite named Haemoproteuscolumbaein the blood of pigeon (Columba livia).
The parasites of genera Haemoproteus
, Plasmodium, and Leucocytozoon are well-known avian haematozoa and can cause declined productivity and high mortality in wild birds.
The prevalence of pigeon parasites was studied on south Khorasan, Iran, were Ascaridia colombae (16.66%), Cotugnia digonopora (13.79%), Raillietina achinobothridia (32.35%), Menopon galline (44.11%), Pseudolynchia canariensis (63.72%), Columbicola colombae (79.41%), Cryptosporidium oocyst (2.94%), Eimeria spp (40.19%) and Haemoproteus