Temperature-Mediated Heteroduplex Analysis
for The Detection of Drug-Resistant Gene Mutations in Clinical Isolates of Mycobacterium tuberculosis by Denaturing HPLC, SURVEYOR Nuclease, Microbes and Infection., 2006; 8:128-135
Fifteen novel FBN1 mutations causing Marfan syndrome detected by heteroduplex analysis
of genomic amplicons.
Study of gene mutation and polymorphism is usually used PCR-SS-CP, manual or automatic sequencing and heteroduplex analysis
, the protein truncation test methods.
Alternate methods can been used to increase the detection sensitivity of melting assays, such as decreasing the PCR product length (10), calibration with an internal oligonucleotide duplex (13), quantitative heteroduplex analysis
after mixing with a known genotype (25), or the use of unlabeled probes (14, 15) or snapback primers (26, 27).
TCR beta variable genes (TCRBV) were amplified, after RNA isolation and cDNA synthesis, using TCRBV-specific primers; then PCR products were subjected to heteroduplex analysis
Identification of bloodmeals in haematophagous Diptera by cytochrome B heteroduplex analysis
(19) A new method, combining seminested polymerase chain reaction with heteroduplex analysis
, was used by Oehadian et al (20) to detect FL cells in peripheral blood.
Identification of mosquito avian-derived blood meals by polymerase chain reaction heteroduplex analysis
. Am J Trop Med Hyg.
Later on, more efficient methods have been discovered including heteroduplex analysis
(Sitte et al., 1995), sequence-specific oligonucleotide typing (Sitte et al., 1996), denaturing gradient gel electrophoresis (Aldridge et al., 1998), and sequencing of the PCR products (Aida et al., 1995).
Methods: The presence of F508del mutation using PCR and heteroduplex analysis
The PCR products were screened by heteroduplex analysis
. Small mutations (<30bp) in the FIX gene were made evident by shifted extra bands.