Gal4-VP16, GTFs, wild-type, and med6 ts mutant Pol II holoenzymes
were prepared using several chromatographic steps as described previously .
Wadzinski, "Positive regulation of Raf1-MEK1/2-ERK1/2 signaling by protein serine/threonine phosphatase 2A holoenzymes
," The Journal of Biological Chemistry, vol.
Furthermore, we observed minimal differences between maximally activated (14/14 subunits) holoenzymes
at 60 nM versus 5 nM (Figures 3(b) and 4(a)--inset, resp.), indicating that while the extent of phosphorylation is sensitive to kinase concentration, changes in the substrate phosphorylation profile are not.
Collagen prolyl hydroxylation involves three isoforms of the P4HA subunit (P4HA1, P4HA2, and P4HA3) that form [A.sub.2][B.sub.2] tetramers with P4HB and eventually P4H1, P4H2, and P4H3 holoenzymes
[90.] Abdulovic AL, Hile SE, Kunkel TA, Eckert KA (2011) The in vitro fidelity of yeast DNA polymerase delta and polymerase epsilon holoenzymes
during dinucleotide microsatellite DNA synthesis.
Each consists of two subunits with estimated molecular weights of 45 ([alpha]) and 25 kDa ([beta]) with molecular masses for the holoenzymes
of approximately 140 kDa, indicative of [[alpha].sub.2][[beta].sub.2] structures.
Most of the reactions in the canonical anabolic chart require enzymes that may be isolated apoenzymes or holoenzymes
(a combination of apoenzymes and coenzymes and/or metal ions).
(16,17) The eukaryotic cell cycle is driven forward by cyclins, which form holoenzymes
together with their cyclin-dependent kinase (CDK) partners.
Comparisons between 11 sequenced bacterial pathogens (Table 1) indicate that approximately 6% of each genome encodes proteins (holoenzymes
and subunits) involved in solute transport.
of cAMP-dependent protein kinase containing the neural form of type I regulatory subunit have an increased sensitivity to cyclic nucleotides.
Protein phosphatase 2A (PP2A) is an important phosphatase which consists of a holoenzyme
composed of the structural A and catalytic C subunits and a regulatory B subunit [1-3].