leucocephala on rumen populations of
holotrich and entodiniomorph, similar data was reported by Sliwinski et al [51] who fed 0.5% and 1.0% CT/kg DM and did not find any effect on these populations in the rumen.
After 12 and 24 h incubation, both
holotrich and entodinomorphid counts were lower in SEQ and WPQ than SQ (P < 0.05) (Table 2).
Exocellular carbohydrase formation by rumen
holotrich ciliates.
Los Molinos (near Valdivia), Chile Lucernaria janetae 8[degrees] 37' North on the East Pacific Rise Table 2 Size and distribution of nematocysts of Lucernaria janetae Tissue Nematocyst n N Range Subumbrellar vesicle Eurytele B, C 34 3/3 19.3-12.2X6.2-8.9
Holotrich A 34 3/3 20.4-16.4X2.2-4.3 Tentacle Eurytele B, C 34 3/3 15.5-12.6X6.4-7.7
Holotrich A 34 3/3 21.8-18.3X2.9-4.5 Gastric filament Eurytele B, C 31 3/3 12.1-10.8X8-8.8 Letters refer to Figure 4; "N" is the proportion of examined specimens that had a particular type of nematocyst; "n" is the number of capsules measured; size presented as range of lengths by widths, in micrometers, for undischarged capsules.
The population of bacteria,
holotrich protozoa, entodiniomorph protozoa and total protozoa counts were unaltered by dietary treatments (p>0.05)
Holotrich protozoa have amylase activity in sufficiently large quantities for using starch as energy source (Kamra, 2005) and protozoa do not appear to produce lactic acid from starch in contrast to rumen bacteria (Van Soest, 1994).
The protozoal population in the inoculum (3.42 x [10.sup.5] cells/mL) consisted of large
holotrich (0.13 x [10.sup.5] cells/mL), small
holotrich (0.5 x [10.sup.5] cells/mL) and small entodiniomorphs (2.79 x [10.sup.5] cells/mL).
The lowest total ciliate protozoal count was observed just before feeding in all the experimental groups, followed by an abrupt increase in the number of
holotrich as well as spirotrich protozoa at 3 h post-feeding in supplemented grounds whereas the protozoal number gradually increased up to 6 h post-feeding in the lambs of unsupplemented group.
The protozoa were assessed as total count only without attempting to differentiate between the various species, although it was apparent that in most samples the entodiniomorphs were the dominant species followed by
Holotrich.