Given the number of hits, this library can be panned under unprecedented aggressive conditions, recovering hundreds of subnanomolar binders in under a week, recovering saturating coverage of hits against every epitope tested, and isolating multi-species cross-reactive members against target homologs
without additional engineering.
In searching GenBank, homologs
of BmARM-like protein were found in many insects, including Papilio xuthus (KPI94899.1), Papilio Machaon (XP_014368349.1), Amyelois transitella (XP_013191284.1), Plutella xylostella (XP_011560493.1), Danaus plexippus (EHJ73656.1), Operophtera brumata (KOB71817.1), Athalia rosae (XP_012263420.1), Aedes aegypti (XP_001650042.1), Culex quinquefasciatus (XP_001843480.1), Anopheles gambiae (XP_319205.3), Anopheles sinensis (KFB52334.1), and Habropoda laboriosa (KOC68595.1).
Two proteins are remote homologs
when they are structurally and functionally related but at the same time they keep a low sequence identity.
2002) obtained from the National Center for Biotechnology Information (NCBI) was used as a query sequence to search homologs
in the common bean (Andean genotype G19833) genome (http://www.phytozome.org) .
Formerly identified immunodominant MAP-derived peptides of a cation transporter [MAP3865c.sub.133-141] (LAANFVVAL) and [MAP3865c.sub.125-133] (MIAVALAGL),a hypothetical protein [MAP2404c.sub.70-85] (RGFVVLPVTRRDVTDV), and a glucan branching protein [MAP1,4[alpha]gbp.sub.157-173] (GTVELLGGPLAHPFQPL) along with their respective homologs
[ZnT8.sub.186-194] (VAANIVLTV), [ZnT8.sub.178-186] (MIIVSSCAV), [PI.sub.46-61] (RGFFYTPKTRREAEDL), and [PI.sub.64-80] (GQVELGGGPGAGSLQPL) [8, 28] were synthesized at >85% purity (LifeTein, South Plainfield, NJ 07080, USA) assessed by HPLC.
Identification of rodent homologs
of hepatitis C virus and pegiviruses.
In this study, a comparative genomics approach was employed to identify Arabidopsis thaliana homologs
of yeast genes involved in UPR.
BACE1 and BACE2 are close homologs
that not only share sequence similarity, but also have very similar 3D structure.
To determine the best homologs
to listeriolysin O (LLO) we performed a blastp search against the Protein Data Bank database (http://www.rcsb.org) at http://www.ncbi.nlm .nih.gov/blast/Blast.cgi.
The genes encoding the two [beta] subunit homologs
were also amplified from the P.
For the phylogenetic comparison, amino acid sequences of different CONSTANS-like homologs
were obtained from GenBank (http://www.