The Fha inactivation probably resulted from changes within the homopolymeric
G tract (site: 1078-1087) from 10 Gs to 11 Gs in fhaB, resulting in a downstream stop codon that produces a truncated FhaB protein (11).
In common with other homopolymeric
C tracts in mtDNA , when the unbroken tract exceeds 11 bp, it generates heteroplasmic length variation.
technology for MiSeq offers slightly better detection of homopolymeric
Any variant that is detected in a homopolymeric
region is verified, with close attention to the corresponding negative control sample to ensure that reported frequency of the variant is adequately above background signal (noise) detected within the region of interest.
It is linear copolymer with homopolymeric
blocks of (1-4)-linked [beta]-D-mannuronate (M) and its C-5 epimer a-L-guluronate (G) residues, respectively.
Meningitides in such a way that the gene encoding lipopolysaccharide glucose transferase enzyme loses a single cystidine residue from homopolymeric
tract of 12 Cs present in coding region of the gene that inturn results in ON switch of the mentioned gene by converting the open reading frame from out-of-frame to in-frame.
Sodium alginate (SA), a natural polysaccharide extracted from seaweed and comprised homopolymeric
blocks of guluronate blocks and mannuronate blocks, was used extensively as supported adsorbent owing to its advantages of biocompatibility, low toxic properties and budget, and ease of gelation [10, 11].
Considering that the sequencing site K1637K is in the poly A position and that pyrosequencing has difficulty in reading the sequence in homopolymeric
regions of the same base in DNA samples [20, 21], we optimized the dispensing order of dNTPs to ensure typing accuracy and sensitivity.
Online Supplemental Table 2 shows that SNV-PER and INDEL >2-bp-PER were associated with specific sequence features, such as amplicon length, GC content, homopolymeric
sequence, and SNP adjacency.
Of each resulting product, 10 [micro]l was pretreated at 95[degrees]C for 5 min, and the denatured DNA was subjected to 3'- end addition of a homopolymeric
C tail using terminal deoxynucleotidyl transferase (TaKaRa) according to the manufacturer's instructions.
These two blocks exist in SA chains as homopolymeric
MM and GG blocks and heteropolymeric MG or GM blocks .
The macromolecular chain of sodium alginate contains both homopolymeric
blocks and blocks of alternating monomeric particles sequences.